Anti-Hu CD152 Alexa Fluor® 647

Anti-Hu CD152 Alexa Fluor® 647
Regulatory status
RUO
Antigen
CD152
Clone
BNI3
Format
Alexa Fluor® 647
Reactivity
Human
Application
FC (QC tested), ICC
Excitation laser
red (633 nm)
Variant
100 tests
A6-785-T100
Out of stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody BNI3 recognizes an extracellular domain of human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.
Application
FC (QC tested), ICC
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human CD152-IgG heavy chain fusion protein
Other names
CTLA4, GSE, GRD4
Preparation
The purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
A6-785_FC_Profil Značení
Flow cytometry surface staining pattern of human PHA stimulated peripheral whole blood stained using anti-human CD152 (BNI3) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).
A6-785_FC_Dot-plot
Flow cytometry multicolor surface staining of human PHA stimulated lymphocytes stained using anti-human CD152 (BNI3) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
A6-785_FC_Histogram
Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD152 (BNI3) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Steiner K, Waase I, Rau T, Dietrich M, Fleischer B, Bröker BM: Enhanced expression of CTLA-4 (CD152) on CD4+ T cells in HIV infection. Clin Exp Immunol. 1999 Mar;115(3):451-7.
PubMed
Rissiek A, Baumann I, Cuapio A, Mautner A, Kolster M, Arck PC, Dodge-Khatami A, Mittrücker HW, Koch-Nolte F, Haag F, Tolosa E: The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation. J Autoimmun. 2015 Apr;58:12-20.
PubMed
Kraszula L, Eusebio M, Kupczyk M, Kuna P, Pietruczuk M: The use of multi-color flow cytometry for identification of functional markers of nTregs in patients with severe asthma. Pneumonol Alergol Pol. 2012;80(5):389-401.
PubMed
Chin LT, Chu C, Chen HM, Hsu SC, Weng BC, Chu CH: Site-directed in vitro immunization leads to a complete human monoclonal IgG4 lambda that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands. BMC Biotechnol. 2007 Aug 23;7:51.
PubMed
Steiner K, Moosig F, Csernok E, Selleng K, Gross WL, Fleischer B, Bröker BM: Increased expression of CTLA-4 (CD152) by T and B lymphocytes in Wegener's granulomatosis. Clin Exp Immunol. 2001 Oct;126(1):143-50.
PubMed
Variant
100 tests
A6-785-T100
Out of stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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