Anti-Hu CD151 APC

Anti-Hu CD151 APC
Regulatory status
RUO
Antigen
CD151
Clone
50-6
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-149-T100
In stock
242.00 USD
Variant
100 tests
1A-149-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody CD151 recognizes an extracellular epitope of CD151 (also known as PETA-3), a 29 kDa transmembrane protein of tetraspanin family, expressed in many cell types.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human epidermoid carcinoma cell line Hep-3
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
RAPH blood group, MER2, SFA1, PETA-3, GP27
Antigen description
CD151, also known as PETA-3 (platelet-endothelial tetraspan antigen), is a four-pass transmembrane glycoprotein with short cytoplasmic N- and C-termini. CD151 is expressed mainly in platelets and megakaryocytes, immature hematopoietic cells, activated T cells, in endothelium, muscle cells, and epithelial cells. It associates with CD9, CD181, and integrin complexes alpha 3 / beta 1 (CD49c / CD29), alpha 5 / beta 1 (CD49e / CD29), and alpha 6 / beta 4 (CD49f / CD104). CD151 appears to be involved in cell adhesion and migration, including metastasis.
Entrez Gene ID 977
UniProt ID P48509
1A-149_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD151 (50-6) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-149_FC_Histogram
Separation of human thrombocytes (red-filled) from CD151 negative neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD151 (50-6) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Sadej R, Grudowska A, Turczyk L, Kordek R, Romanska HM: CD151 in cancer progression and metastasis: a complex scenario. Lab Invest. 2014 Jan;94(1):41-51.
PubMed
Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, Anstee DJ: Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1. PLoS One. 2013 May 21;8(5):e62654.
PubMed
Variant
100 tests
1A-149-T100
In stock
242.00 USD
Variant
100 tests
1A-149-T100
In stock
242.00 USD

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