Anti-Hu CD15 Purified

Anti-Hu CD15 Purified
Regulatory status
RUO
Antigen
CD15
Clone
W6D3
Format
Purified
Reactivity
Human
Application
FC (QC tested)
Variant
0.1 mg
11-864-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
Mouse monoclonal antibody W6D3 recognizes an extracellular epitope of CD15, a cell membrane 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. This is a non-IgM anti-CD15 antibody.
Workshop
HLDA VIII
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
WERI-RB-1 retinoblastoma cell line
Other names
Lewis x Blood Group antigen, Le(x), SSEA-1, 3-fucosyl-N-acetyllactosamine
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-864_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD15 (W6D3) purified antibody (concentration in sample 15 μg/ml, GAM APC).
11-864_FC_Histogram
Separation of neutrophil granulocytes stained anti-human CD15 (W6D3) purified antibody (concentration in sample 15 μg/ml, GAM APC, red-filled) from neutrophil granulocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

General references:

Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep;39(1-2):185-94.
PubMed
Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov;24(11):771-6.
PubMed
Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1;406(2):333-41.
PubMed

Product specific references:

Mandalasi M, Dorabawila N, Smith DF, Heimburg-Molinaro J, Cummings RD, Nyame AK: Development and characterization of a specific IgG monoclonal antibody toward the Lewis x antigen using splenocytes of Schistosoma mansoni-infected mice. Glycobiology. 2013 Jul;23(7):877-92.
PubMed
Sutherland DR, Ortiz F, Quest G, Illingworth A, Benko M, Nayyar R, Marinov I: High-sensitivity 5-, 6-, and 7-color PNH WBC assays for both Canto II and Navios platforms. Cytometry B Clin Cytom. 2018 Jul;94(4):637-651.
PubMed
Variant
0.1 mg
11-864-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD