Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD148 Biotin

Anti-Hu CD148 Biotin

Regulatory status

RUO

Antigen

CD148

Clone

MEM-CD148/05

Format

Biotin

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

1B-697-C100

In stock

198.00 USD

0.025 mg

1B-697-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

1B-697-C100

In stock

198.00 USD

0.025 mg

1B-697-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody MEM-CD148/05 recognizes an extracellular epitope of CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-12 μg/ml.

Reactivity

Human

Immunogen

Human recombinant CD148 (amino acids 1-444)

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

HPTP-eta, DEP-1, PTPRJ, DEP1, SCC1, HPTPeta, R-PTP-ETA

Antigen description

CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase, containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.

Entrez Gene ID 5795

UniProt ID Q12913

Flow cytometry analysis (surface staining) of human peripheral blood leukocytes using anti-CD148 (MEM-CD148/05) biotin / streptavidin-APC.

General references:

Stepanek O, Kalina T, Draber P, Skopcova T, Svojgr K, Angelisova P, Horejsi V, Weiss A, Brdicka T: Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148. J Biol Chem. 2011 Jun 24;286(25):22101-12.
PubMed