Anti-Hu CD148 APC

Anti-Hu CD148 APC
Regulatory status
RUO
Antigen
CD148
Clone
MEM-CD148/05
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-697-T100
In stock
242.00 USD

25 tests
1A-697-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1A-697-T100
In stock
242.00 USD

25 tests
1A-697-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody MEM-CD148/05 recognizes an extracellular epitope of CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human recombinant CD148 (amino acids 1-444)
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
HPTP-eta, DEP-1, PTPRJ, DEP1, SCC1, HPTPeta, R-PTP-ETA
Antigen description
CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase, containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.
Entrez Gene ID 5795
UniProt ID Q12913
1A-697 FC
Flow cytometry analysis (surface staining) of human peripheral blood using anti-CD148 (MEM-CD148/05) APC.
1A-697 FC_2
Flow cytometry analysis (surface staining) of human peripheral blood using anti-CD148 (MEM-CD148/05) APC.

General references:

Stepanek O, Kalina T, Draber P, Skopcova T, Svojgr K, Angelisova P, Horejsi V, Weiss A, Brdicka T: Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148. J Biol Chem. 2011 Jun 24;286(25):22101-12.
PubMed
Variant
100 tests
1A-697-T100
In stock
242.00 USD

25 tests
1A-697-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1A-697-T100
In stock
242.00 USD

25 tests
1A-697-T025
Delivery 1 week
121.00 USD

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