Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD147 Purified Low Endotoxin

Anti-Hu CD147 Purified Low Endotoxin

Anti-Hu CD147 Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD147
Clone
MEM-M6/6
Format
Purified Low Endotoxin
Reactivity
Human
Application
WB, FC (QC tested), FUNC
Variant
0.1 mg
12-507-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Contact distributor
Product details
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-M6/6 recognizes extracellular Ig domain D2 (membrane proximal) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes.
Application
WB, FC (QC tested), FUNC
Application details
Functional application: The antibody MEM-M6/6 (high-affinity mAb of unique epitope specificity) inhibits anti-CD3-induced T cell activation.
Flow cytometry: recommended dilution: 1-4 μg/ml.
Western blotting: Positive control: 293 human fibroblastoid cell line, non-reducing conditions.
Reactivity
Human
Immunogen
Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimmer).
Other names
Neurothelin, Basigin, LAM6, CSF, 5F7, TCSF, BSG, TCSF, Emprin, OK blood group
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

General references:

Kirk P, Wilson MC, Heddle C, Brown MH, Barclay AN, Halestrap AP: CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression. EMBO J. 2000 Aug 1;19(15):3896-904.
PubMed
Wilson MC, Meredith D, Fox JE, Manoharan C, Davies AJ, Halestrap AP: Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 2005 Jul 22;280(29):27213-21.
PubMed
Xu D, Hemler ME: Metabolic activation-related CD147-CD98 complex. Mol Cell Proteomics. 2005 Aug;4(8):1061-71.
PubMed
Ruiz S, Castro-Castro A, Bustelo XR: CD147 Inhibits the Nuclear Factor of Activated T-cells by Impairing Vav1 and Rac1 Downstream Signaling. J Biol Chem. 2008 Feb 29;283(9):5554-66.
PubMed
Iacono KT, Brown AL, Greene MI, Saouaf SJ: CD147 immunoglobulin superfamily receptor function and role in pathology. Exp Mol Pathol. 2007 Dec;83(3):283-95.
PubMed
Melchior A, Denys A, Deligny A, Mazurier J, Allain F: Cyclophilin B induces integrin-mediated cell adhesion by a mechanism involving CD98-dependent activation of protein kinase C-delta and p44/42 mitogen-activated protein kinases. Exp Cell Res. 2008 Feb 1;314(3):616-28.
PubMed
Schmidt R, Bültmann A, Fischel S, Gillitzer A, Cullen P, Walch A, Jost P, Ungerer M, Tolley ND, Lindemann S, Gawaz M, Schömig A, May AE. Extracellular matrix metalloproteinase inducer (CD147) is a novel receptor on platelets, activates platelets, and augments nuclear factor kappaB-dependent inflammation in monocytes. Circ Res. 2008 Feb 15;102(3):302-9.
PubMed

Product specific references:

Somno A, Anuchapreeda S, Chruewkamlow N, Pata S, Kasinrerk W, Chiampanichayakul S: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line. Leuk Res Rep. 2016 Aug 26;6:33-8
PubMed
Staffler G, Szekeres A, Schütz GJ, Säemann MD, Prager E, Zeyda M, Drbal K, Zlabinger GJ, Stulnig TM, Stockinger H: Selective inhibition of T cell activation via CD147 through novel modulation of lipid rafts. J Immunol. 2003 Aug 15;171(4):1707-14.
PubMed
Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11(5):777-86.
PubMed
Variant
0.1 mg
12-507-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Contact distributor
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