Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD144 PE-Cy™7

Anti-Hu CD144 PE-Cy™7

Anti-Hu CD144 PE-Cy™7
Regulatory status
RUO
Antigen
CD144
Clone
55-7H1
Format
PE-Cy™7
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
T7-770-T100
In stock
250.00 USD
Variant
0.1 mg
11-261-C100
In stock
250.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.
Workshop
HLDA X
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human endothelial cells
Preparation
The purified antibody is conjugated with tandem dye PE-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
Cadherin 5, CDH5, 7B4, VE-cadherin
Antigen description
CD144 / VE-cadherin (cadherin 5) is the major cadherin that is present at endothelial junctions. It is also strictly endothelial specific. Under vascular permeability increasing conditions (and also in capillaries and veins) CD144 is being phosphorylated, which promotes its rapid and reversible internalization. On the contrary, binding of p120 catenin (delta1 catenin) maintains CD144 localization at the plasma membrane, which stabilizes the junction and reduces vascular permeability.
Entrez Gene ID 1003
UniProt ID P33151
T7-770_FC_Histogram
Separation of HUVEC cells (red-filled) from HL-60 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD144 (55-7H1) PE-Cy™7 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

General references:

Dejana E, Orsenigo F: Endothelial adherens junctions at a glance. J Cell Sci. 2013 Jun 15;126(Pt 12):2545-9.
PubMed

Product specific references:

Tisato V, Zauli G, Voltan R, Gianesini S, di Iasio MG, Volpi I, Fiorentini G, Zamboni P, Secchiero P: Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype. PLoS One. 2012;7(6):e39543.
PubMed
Agrawal V, Kelly J, Tottey S, Daly KA, Johnson SA, Siu BF, Reing J, Badylak SF: An isolated cryptic peptide influences osteogenesis and bone remodeling in an adult mammalian model of digit amputation. Tissue Eng Part A. 2011 Dec;17(23-24):3033-44.
PubMed
De Ugarte DA, Alfonso Z, Zuk PA, Elbarbary A, Zhu M, Ashjian P, Benhaim P, Hedrick MH, Fraser JK: Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow. Immunol Lett. 2003 Oct 31;89(2-3):267-70.
PubMed
Limaye V, Li X, Hahn C, Xia P, Berndt MC, Vadas MA, Gamble JR: Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members. Blood. 2005 Apr 15;105(8):3169-77
PubMed
Hamilton RD, Foss AJ, Leach L: Establishment of a human in vitro model of the outer blood-retinal barrier. J Anat. 2007 Dec;211(6):707-16
PubMed
Variant
100 tests
T7-770-T100
In stock
250.00 USD
Variant
0.1 mg
11-261-C100
In stock
250.00 USD
Contact distributor
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SDS download
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Technical support for this product