Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD144 PE-Cy™7

Anti-Hu CD144 PE-Cy™7

Regulatory status

RUO

Antigen

CD144

Clone

55-7H1

Format

PE-Cy™7

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-770-T100

In stock

250.00 USD

Variant

100 tests

T7-770-T100

In stock

250.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.

Workshop

HLDA VI: WS code E092

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human endothelial cells

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

Cadherin 5, CDH5, 7B4, VE-cadherin

Antigen description

CD144 / VE-cadherin (cadherin 5) is the major cadherin that is present at endothelial junctions. It is also strictly endothelial specific. Under vascular permeability increasing conditions (and also in capillaries and veins) CD144 is being phosphorylated, which promotes its rapid and reversible internalization. On the contrary, binding of p120 catenin (delta1 catenin) maintains CD144 localization at the plasma membrane, which stabilizes the junction and reduces vascular permeability.

Entrez Gene ID 1003

UniProt ID P33151

Separation of HUVEC cells (red-filled) from HL-60 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD144 (55-7H1) PE-Cy™7 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

General references:

Dejana E, Orsenigo F: Endothelial adherens junctions at a glance. J Cell Sci. 2013 Jun 15;126(Pt 12):2545-9.
PubMed

Product specific references:

Tisato V, Zauli G, Voltan R, Gianesini S, di Iasio MG, Volpi I, Fiorentini G, Zamboni P, Secchiero P: Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype. PLoS One. 2012;7(6):e39543.
PubMed

Agrawal V, Kelly J, Tottey S, Daly KA, Johnson SA, Siu BF, Reing J, Badylak SF: An isolated cryptic peptide influences osteogenesis and bone remodeling in an adult mammalian model of digit amputation. Tissue Eng Part A. 2011 Dec;17(23-24):3033-44.
PubMed

De Ugarte DA, Alfonso Z, Zuk PA, Elbarbary A, Zhu M, Ashjian P, Benhaim P, Hedrick MH, Fraser JK: Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow. Immunol Lett. 2003 Oct 31;89(2-3):267-70.
PubMed

Limaye V, Li X, Hahn C, Xia P, Berndt MC, Vadas MA, Gamble JR: Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members. Blood. 2005 Apr 15;105(8):3169-77
PubMed

Hamilton RD, Foss AJ, Leach L: Establishment of a human in vitro model of the outer blood-retinal barrier. J Anat. 2007 Dec;211(6):707-16
PubMed