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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD144 Alexa Fluor<sup>®</sup> 647
Anti-Hu CD144 Alexa Fluor
®
647
Regulatory status
RUO
Antigen
CD144
Clone
55-7H1
Format
Alexa Fluor
®
647
Reactivity
Human
Application
WB, FC (QC tested), ICC
Excitation laser
red (633 nm)
Variant
100 tests
A6-770-T100
In stock
242.00 USD
Variant
100 tests
A6-770-T100
In stock
242.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.
Workshop
HLDA VI: WS code E092
Application
WB, FC (QC tested), ICC
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human endothelial cells
Preparation
Purified antibody is conjugated with Alexa Fluor
®
647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor
®
, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
Cadherin 5, CDH5, 7B4, VE-cadherin
Antigen description
CD144 / VE-cadherin (cadherin 5) is the major cadherin that is present at endothelial junctions. It is also strictly endothelial specific. Under vascular permeability increasing conditions (and also in capillaries and veins) CD144 is being phosphorylated, which promotes its rapid and reversible internalization. On the contrary, binding of p120 catenin (delta1 catenin) maintains CD144 localization at the plasma membrane, which stabilizes the junction and reduces vascular permeability.
Entrez Gene ID
1003
UniProt ID
P33151
Separation of HUVEC cells (red-filled) from HL-60 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD144 (55-7H1) Alexa Fluor® 647 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).
General references:
Dejana E, Orsenigo F: Endothelial adherens junctions at a glance. J Cell Sci. 2013 Jun 15;126(Pt 12):2545-9.
PubMed
Product specific references:
Tisato V, Zauli G, Voltan R, Gianesini S, di Iasio MG, Volpi I, Fiorentini G, Zamboni P, Secchiero P: Endothelial cells obtained from patients affected by chronic venous disease exhibit a pro-inflammatory phenotype. PLoS One. 2012;7(6):e39543.
PubMed
Agrawal V, Kelly J, Tottey S, Daly KA, Johnson SA, Siu BF, Reing J, Badylak SF: An isolated cryptic peptide influences osteogenesis and bone remodeling in an adult mammalian model of digit amputation. Tissue Eng Part A. 2011 Dec;17(23-24):3033-44.
PubMed
De Ugarte DA, Alfonso Z, Zuk PA, Elbarbary A, Zhu M, Ashjian P, Benhaim P, Hedrick MH, Fraser JK: Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow. Immunol Lett. 2003 Oct 31;89(2-3):267-70.
PubMed
Limaye V, Li X, Hahn C, Xia P, Berndt MC, Vadas MA, Gamble JR: Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members. Blood. 2005 Apr 15;105(8):3169-77
PubMed
Hamilton RD, Foss AJ, Leach L: Establishment of a human in vitro model of the outer blood-retinal barrier. J Anat. 2007 Dec;211(6):707-16
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
A6-770-T100
In stock
242.00 USD
Variant
100 tests
A6-770-T100
In stock
242.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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