Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD143 PE

Anti-Hu CD143 PE

Regulatory status

RUO

Antigen

CD143

Clone

5-369

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-796-T100

In stock

242.00 USD

25 tests

1P-796-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-796-T100

In stock

242.00 USD

25 tests

1P-796-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 5-369 recognizes an extracellular epitope of CD143, a 171 kDa type I transmembrane glycoprotein with metallopeptidase activity, expressed mainly on endothelial cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

dendritic cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DCP, ACE1, DCP1, carboxycathepsin, kininase II, peptidase P, peptidyl dipeptidase 1

Antigen description

CD143, also known as ACE (angiotensin-converting enzyme), carboxycathepsin, kininase II, peptidase P, or peptidyl dipeptidase 1, is a transmembrane zinc metallopeptidase catalyzing the conversion of angiotensin I into the physiologically active angiotensin II, which is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, that are equally active. CD143 is expressed mainly on endothelial cells, but it can be found also e.g. on activated macrophages and histiocytes.

Entrez Gene ID 1636

UniProt ID P12821