Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD142 Purified

Anti-Hu CD142 Purified

Regulatory status

RUO

Antigen

CD142

Clone

HTF-1

Format

Purified

Reactivity

Human

Application

WB, FC (QC tested), IHC(P), ICC

Variant

0.1 mg

11-130-C100

In stock

143.00 USD

0.025 mg

11-130-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-130-C100

In stock

143.00 USD

0.025 mg

11-130-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody HTF-1, also known as HTF1-7B8, recognizes an extracellular epitope of CD142 (tissue factor, coagulation factor III), a type I glycoprotein expressed on endothelial cells, monocytes, macrophages, and platelets upon induction by inflammatory mediators, and expressed constitutively by some tumors, the vasculature, placenta, kidney, and central nervous system.

Workshop

HLDA VI: WS Code E016

Application

WB, FC (QC tested), IHC(P), ICC

Application details

Flow cytometry: Recommended dilution: 3-12 µg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Human

Immunogen

Human brain tissue factor (CD142)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

F3, tissue factor, tissue thromboplastin, coagulation factor III, TF, TFA

Antigen description

CD142, also known as coagulation factor III, tissue thromboplastin, and tissue factor. It is a transmembrane glycoprotein, which enables cells to initiate the blood coagulation cascades, and functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. It is the only one factor in the coagulation pathway for which a congenital deficiency has not been described.

Entrez Gene ID 2152

UniProt ID P13726

Flow cytometry multicolor surface staining pattern of human PHA stimulated peripheral blood mononuclear cells using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC) and SYTO 16.

Separation of human CD142 positive lymphocytes (red-filled) from CD142 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD142 (HTF-1) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Immunohistochemistry staining of human cortex (paraffin-embedded sections) with anti-CD142 (HTF-1), 10 μg/ml. Commercially tested by LifeSpan BioSciences.

Western blotting analysis of human CD142 using mouse monoclonal antibody HTF-1 on lysates of A431 cell line and MCF7 and THP1 cell lines (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD142 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD142 was detected around 50 kDa.

Product specific references:

Bucciarelli P, Martinelli I, Artoni A, Passamonti SM, Previtali E, Merati G, Tripodi A, Mannucci PM: Circulating microparticles and risk of venous thromboembolism. Thromb Res. 2012 May;129(5):591-7
PubMed

Patil R, Ghosh K, Satoskar P, Shetty S: Elevated procoagulant endothelial and tissue factor expressing microparticles in women with recurrent pregnancy loss. PLoS One. 2013 Nov 20;8(11):e81407.
PubMed

Aass HC, Øvstebø R, Trøseid AM, Kierulf P, Berg JP, Henriksson CE: Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis. Cytometry A. 2011 Dec;79(12):990-9. doi: 10.1002/cyto.a.21147.
PubMed

Carson SD, Ross SE, Bach R, Guha A: An inhibitory monoclonal antibody against human tissue factor. Blood. 1987 Aug;70(2):490-3.
PubMed

Faulk WP, Labarrere CA, Carson SD: Tissue factor: Identification and characterization of cell types in human placentae. Volume 76, Issue 1, 1 July 1990, Pages 86-96.
PubMed

Virtanen S, Schulte R, Stingl J, Caldas C, Shehata M: High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity. Breast Cancer Res. 2021 Jun 13;23(1):66.
PubMed