Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD142 PE

Anti-Hu CD142 PE

Regulatory status

RUO

Antigen

CD142

Clone

HTF-1

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-130-T100

In stock

220.00 USD

25 tests

1P-130-T025

In stock

110.00 USD

Variant

100 tests

1P-130-T100

In stock

220.00 USD

25 tests

1P-130-T025

In stock

110.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody HTF-1, also known as HTF1-7B8, recognizes an extracellular epitope of CD142 (tissue factor, coagulation factor III), a type I glycoprotein expressed on endothelial cells, monocytes, macrophages, and platelets upon induction by inflammatory mediators, and expressed constitutively by some tumors, the vasculature, placenta, kidney, and central nervous system.

Workshop

HLDA VI: WS Code E016

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human brain tissue factor (CD142)

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

F3, tissue factor, tissue thromboplastin, coagulation factor III, TF, TFA

Antigen description

CD142, also known as coagulation factor III, tissue thromboplastin, and tissue factor. It is a transmembrane glycoprotein, which enables cells to initiate the blood coagulation cascades, and functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. It is the only one factor in the coagulation pathway for which a congenital deficiency has not been described.

Entrez Gene ID 2152

UniProt ID P13726

Separation of A431 cells (red-filled) from Jurkat cells (black-dashed) in flow cytometry analysis (surface staining) of cellular suspension stained using anti-human CD142 (HTF-1) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension).

Product specific references:

Bucciarelli P, Martinelli I, Artoni A, Passamonti SM, Previtali E, Merati G, Tripodi A, Mannucci PM: Circulating microparticles and risk of venous thromboembolism. Thromb Res. 2012 May;129(5):591-7
PubMed

Patil R, Ghosh K, Satoskar P, Shetty S: Elevated procoagulant endothelial and tissue factor expressing microparticles in women with recurrent pregnancy loss. PLoS One. 2013 Nov 20;8(11):e81407.
PubMed

Aass HC, Øvstebø R, Trøseid AM, Kierulf P, Berg JP, Henriksson CE: Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis. Cytometry A. 2011 Dec;79(12):990-9. doi: 10.1002/cyto.a.21147.
PubMed

Carson SD, Ross SE, Bach R, Guha A: An inhibitory monoclonal antibody against human tissue factor. Blood. 1987 Aug;70(2):490-3.
PubMed

Faulk WP, Labarrere CA, Carson SD: Tissue factor: Identification and characterization of cell types in human placentae. Volume 76, Issue 1, 1 July 1990, Pages 86-96.
PubMed

Virtanen S, Schulte R, Stingl J, Caldas C, Shehata M: High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity. Breast Cancer Res. 2021 Jun 13;23(1):66.
PubMed