Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD141 Purified

Anti-Hu CD141 Purified

Regulatory status

RUO

Antigen

CD141

Clone

M80

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-795-C100

In stock

154.00 USD

Variant

0.1 mg

11-795-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody M80 recognizes an extracellular epitope of CD141, a 75 kDa transmembrane glycoprotein expressed mainly on macrophages, monocytes, platelets and endothelial cells.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 µg/ml

Reactivity

Human

Immunogen

MV4-11 cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Thrombomodulin, fetomodulin, THBD, THRM, THPH12, TM

Antigen description

CD141, also known as thrombomodulin or fetomodulin, is a single chain type I transmembrane glycoprotein serving as a receptor for thrombin and as an important cofactor in the protein C anticoagulant system, but it is also involved in embryonic and atherosclerotic plaque development. CD141 is expressed mainly on macrophages, monocytes, a subpopulation of myeloid dendritic cells, on platelets and endothelial cells, but also e.g. on keratinocytes (epithelium). After binding to thrombin, CD141 activates protein C, which degrades clotting factors Va and VIIIa, and as a consequence the amount of thrombin is reduced. Mutations in the CD141 gene can cause a thromboembolic disease known as inherited thrombophilia.

Entrez Gene ID 7056

UniProt ID P07204

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD141 (M80) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD141 (M80) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Product specific references:

Jenner W, Motwani M, Veighey K, Newson J, Audzevich T, Nicolaou A, Murphy S, Macallister R, Gilroy DW: Characterisation of leukocytes in a human skin blister model of acute inflammation and resolution. PLoS One. 2014 Mar 6;9(3):e89375
PubMed