Anti-Hu CD141 Purified

Anti-Hu CD141 Purified
Regulatory status
RUO
Antigen
CD141
Clone
M80
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-795-C100
In stock
154.00 USD
Variant
0.1 mg
11-795-C100
In stock
154.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody M80 recognizes an extracellular epitope of CD141, a 75 kDa transmembrane glycoprotein expressed mainly on macrophages, monocytes, platelets and endothelial cells.
Application
Application details
Flow cytometry: Recommended dilution: 1-5 µg/ml
Reactivity
Human
Immunogen
MV4-11 cell line
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Thrombomodulin, fetomodulin, THBD, THRM, THPH12, TM
Antigen description
CD141, also known as thrombomodulin or fetomodulin, is a single chain type I transmembrane glycoprotein serving as a receptor for thrombin and as an important cofactor in the protein C anticoagulant system, but it is also involved in embryonic and atherosclerotic plaque development. CD141 is expressed mainly on macrophages, monocytes, a subpopulation of myeloid dendritic cells, on platelets and endothelial cells, but also e.g. on keratinocytes (epithelium). After binding to thrombin, CD141 activates protein C, which degrades clotting factors Va and VIIIa, and as a consequence the amount of thrombin is reduced. Mutations in the CD141 gene can cause a thromboembolic disease known as inherited thrombophilia.
Entrez Gene ID 7056
UniProt ID P07204
11-795_FC_Histogram
Anti-Hu CD141 purified antibody (clone M80) specificity verification by flow cytometry

Anti-Hu CD141 purified antibody (concentration in sample 0.3 μg/ml, GAM APC, red-filled histogram) binds specifically to CD141 on surface of HUVEC cells with high expression of CD141 (upper panel) and also TF-1 cells with lower CD141 expresion (middle panel), but not to surface of K562 cells non-expressing CD141 (lower panel). Level of non-specific binding was assessed using cells unstained by primary antibody (GAM APC, black-dashed histogram).

Product specific references:

Jenner W, Motwani M, Veighey K, Newson J, Audzevich T, Nicolaou A, Murphy S, Macallister R, Gilroy DW: Characterisation of leukocytes in a human skin blister model of acute inflammation and resolution. PLoS One. 2014 Mar 6;9(3):e89375
PubMed
Variant
0.1 mg
11-795-C100
In stock
154.00 USD
Variant
0.1 mg
11-795-C100
In stock
154.00 USD

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