Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD141 PE

Anti-Hu CD141 PE

Regulatory status

RUO

Antigen

CD141

Clone

M80

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-795-T100

In stock

242.00 USD

Variant

100 tests

1P-795-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody M80 recognizes an extracellular epitope of CD141, a 75 kDa transmembrane glycoprotein expressed mainly on macrophages, monocytes, platelets and endothelial cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

MV4-11 cell line

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Thrombomodulin, fetomodulin, THBD, THRM, THPH12, TM

Antigen description

CD141, also known as thrombomodulin or fetomodulin, is a single chain type I transmembrane glycoprotein serving as a receptor for thrombin and as an important cofactor in the protein C anticoagulant system, but it is also involved in embryonic and atherosclerotic plaque development. CD141 is expressed mainly on macrophages, monocytes, a subpopulation of myeloid dendritic cells, on platelets and endothelial cells, but also e.g. on keratinocytes (epithelium). After binding to thrombin, CD141 activates protein C, which degrades clotting factors Va and VIIIa, and as a consequence the amount of thrombin is reduced. Mutations in the CD141 gene can cause a thromboembolic disease known as inherited thrombophilia.

Entrez Gene ID 7056

UniProt ID P07204

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD141 (M80) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD141 (M80) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Jenner W, Motwani M, Veighey K, Newson J, Audzevich T, Nicolaou A, Murphy S, Macallister R, Gilroy DW: Characterisation of leukocytes in a human skin blister model of acute inflammation and resolution. PLoS One. 2014 Mar 6;9(3):e89375
PubMed