Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD140b Purified

Anti-Hu CD140b Purified

Regulatory status

RUO

Antigen

CD140b

Clone

18A2

Format

Purified

Reactivity

Human

Application

FC (QC tested), ICC

Variant

0.1 mg

11-590-C100

In stock

165.00 USD

0.025 mg

11-590-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-590-C100

In stock

165.00 USD

0.025 mg

11-590-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The monoclonal antibody 18A2 recognizes an extracellular epitope of CD140b / PDGF-RB, the 180-190 kDa beta chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative roles.

Application

FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

CD140b-transfected NIH 3T3 cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PDGFRB, PDGFR1

Antigen description

CD140b / PDGF-RB (platelet-derived growth factor receptor beta) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140b forms homodimers, or heterodimerizes with CD140a / PDGF-RA. Whereas CD140a can have both pro-proliferative or anti-proliferative effects, the CD140b induces in various cell types their proliferation and migration. CD140b has also developmental roles in the cardiovascular system and is preferentially expressed on some tumours such as medulloblastoma.

Entrez Gene ID 5159

UniProt ID Q59F04

Anti-Hu CD140b purified antibody (clone 18A2) specificity verification by flow cytometry

Anti-Hu CD140b purified antibody (concentration in sample 6 μg/ml, GAM APC, red-filled histogram) binds specifically to CD140b on surface of CD140b transfected cells (upper panel), but not to 3T3 cells (lower panel). Level of non-specific binding was assessed using cells unstained by primary antibody (GAM APC, black-dashed histogram).

General references:

Andrae J, Gallini R, Betsholtz C: Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 2008 May 15;22(10):1276-312.
PubMed

French WJ, Creemers EE, Tallquist MD: Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol. 2008 Sep;28(18):5646-57.
PubMed

Schmahl J, Rizzolo K, Soriano P: The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev. 2008 Dec 1;22(23):3255-67.
PubMed

Faraone D, Aguzzi MS, Toietta G, Facchiano AM, Facchiano F, Magenta A, Martelli F, Truffa S, Cesareo E, Ribatti D, Capogrossi MC, Facchiano A: Platelet-derived growth factor-receptor alpha strongly inhibits melanoma growth in vitro and in vivo. Neoplasia. 2009 Aug;11(8):732-42.
PubMed

Product specific references:

Fekete N, Rojewski MT, Fürst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H: GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC. PLoS One. 2012;7(8):e43255. doi: 10.1371/journal.pone.0043255
PubMed