Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD140a Purified

Anti-Hu CD140a Purified

Regulatory status

RUO

Antigen

CD140a

Clone

16A1

Format

Purified

Reactivity

Human

Application

FC (QC tested), ICC

Variant

0.1 mg

11-589-C100

In stock

165.00 USD

0.025 mg

11-589-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-589-C100

In stock

165.00 USD

0.025 mg

11-589-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 16A1 recognizes an extracellular epitope of CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.

Workshop

HLDA VI: WS Code E022

Application

FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 2 μg/ml.

Reactivity

Human

Immunogen

CD140a-transfected NIH 3T3 cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PDGFRA, PDGFR2

Antigen description

CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b / PDGF-RB. Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions.

Entrez Gene ID 5156

UniProt ID P16234

Separation of cells stained using anti-human CD140a (16A1) purified antibody (concentration in sample 0,33 μg/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of CD140a transfected cell suspension.

General references:

Faraone D, Aguzzi MS, Toietta G, Facchiano AM, Facchiano F, Magenta A, Martelli F, Truffa S, Cesareo E, Ribatti D, Capogrossi MC, Facchiano A: Platelet-derived growth factor-receptor alpha strongly inhibits melanoma growth in vitro and in vivo. Neoplasia. 2009 Aug;11(8):732-42.
PubMed

Schmahl J, Rizzolo K, Soriano P: The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev. 2008 Dec 1;22(23):3255-67.
PubMed

French WJ, Creemers EE, Tallquist MD: Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol. 2008 Sep;28(18):5646-57.
PubMed

Andrae J, Gallini R, Betsholtz C: Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 2008 May 15;22(10):1276-312.
PubMed