Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD140a PE-Cy™7

Anti-Hu CD140a PE-Cy™7

Regulatory status

RUO

Antigen

CD140a

Clone

16A1

Format

PE-Cy™7

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-589-T100

Ask for delivery term HERE

275.00 USD

Variant

100 tests

T7-589-T100

Ask for delivery term HERE

275.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 16A1 recognizes an extracellular epitope of CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.

Workshop

HLDA VI: WS Code E022

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CD140a-transfected NIH 3T3 cells

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

PDGFRA, PDGFR2

Antigen description

CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b / PDGF-RB. Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions.

Entrez Gene ID 5156

UniProt ID P16234

Separation of cells stained using anti-human CD140a (16A1) PE-Cy™7 antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) PE-Cy™7 antibody (concentration in sample 3 μg/ml, same as CD140a PE-Cy™7 concentration, black-dashed) in flow cytometry analysis (surface staining) of PDGF-RA transfected cells.

General references:

Faraone D, Aguzzi MS, Toietta G, Facchiano AM, Facchiano F, Magenta A, Martelli F, Truffa S, Cesareo E, Ribatti D, Capogrossi MC, Facchiano A: Platelet-derived growth factor-receptor alpha strongly inhibits melanoma growth in vitro and in vivo. Neoplasia. 2009 Aug;11(8):732-42.
PubMed

Schmahl J, Rizzolo K, Soriano P: The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev. 2008 Dec 1;22(23):3255-67.
PubMed

French WJ, Creemers EE, Tallquist MD: Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol. 2008 Sep;28(18):5646-57.
PubMed

Andrae J, Gallini R, Betsholtz C: Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 2008 May 15;22(10):1276-312.
PubMed