Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD137 Purified Low Endotoxin

Anti-Hu CD137 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD137

Clone

4B4-1

Format

Purified Low Endotoxin

Reactivity

Human, Non-human primates

Application

ELISA, IP, FC (QC tested), FUNC

Variant

0.1 mg

12-143-C100

In stock

143.00 USD

Variant

0.1 mg

12-143-C100

In stock

143.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 4B4-1 recognizes an extracellular conformational epitope on CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.

Workshop

HLDA VI: WS Code C-7

Application

ELISA, IP, FC (QC tested), FUNC

Application details

Functional application: Inhibition of cytokine production.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human, Non-human primates

Immunogen

Recombinant human CD137 ectodomain

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TNFRSF9, 41BB, 4-1BB, MGC2172

Antigen description

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

Entrez Gene ID 3604

UniProt ID Q07011

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD137 (4B4-1) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Separation of cells stained using anti-humam CD137 (4B4-1) purified antibody (low endotoxin, concentration in sample 4 μg/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cell suspension.

Product specific references:

Bellarosa D, Bressan A, Bigioni M, Parlani M, Maggi CA, Binaschi M: SAHA/Vorinostat induces the expression of the CD137 receptor/ligand system and enhances apoptosis mediated by soluble CD137 receptor in a human breast cancer cell line. Int J Oncol. 2012 Oct;41(4):1486-94.
PubMed

Fernández Do Porto DA, Jurado JO, Pasquinelli V, Alvarez IB, Aspera RH, Musella RM, García VE: CD137 differentially regulates innate and adaptive immunity against Mycobacterium tuberculosis. Immunol Cell Biol. 2012 Apr;90(4):449-56.
PubMed

Yi L, Zhao Y, Wang X, Dai M, Hellström KE, Hellström I, Zhang H: Human and mouse CD137 have predominantly different binding CRDs to their respective ligands. PLoS One. 2014 Jan 21;9(1):e86337.
PubMed

Chan SL, Voskens CJ, Lin W, Schindler DG, Azimzadeh A, Wang LX, Taylor RJ, Strome SE, Schulze DH: Epitope mapping of a chimeric CD137 mAb: a necessary step for assessing the biologic relevance of non-human primate models. J Mol Recognit. 2009 May-Jun;22(3):242-9. doi: 10.1002/jmr.937.
PubMed

Kienzle G, von Kempis J: CD137 (ILA/4-1BB), expressed by primary human monocytes, induces monocyte activation and apoptosis of B lymphocytes. Int Immunol. 2000 Jan;12(1):73-82.
PubMed

Salih HR, Kosowski SG, Haluska VF, Starling GC, Loo DT, Lee F, Aruffo AA, Trail PA, Kiener PA: Constitutive expression of functional 4-1BB (CD137) ligand on carcinoma cells. J Immunol. 2000 Sep 1;165(5):2903-10.
PubMed