Anti-Hu CD137 APC

Anti-Hu CD137 APC
Regulatory status
RUO
Antigen
CD137
Clone
4B4-1
Format
APC
Reactivity
Human, Non-human primates
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-143-T100
In stock
242.00 USD
Variant
100 tests
1A-143-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 4B4-1 recognizes an extracellular conformational epitope on CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.
Workshop
HLDA VI: WS Code C-7
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-human primates
Immunogen
Recombinant human CD137 ectodomain
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TNFRSF9, 41BB, 4-1BB, MGC2172
Antigen description
CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.
Entrez Gene ID 3604
UniProt ID Q07011
1A-143_FC_Profil Značení
Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD137 (4B4-1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1A-143_FC_Dot-plot
Flow cytometry multicolor surface staining of human peripheral blood mononulcear cells stained using anti-humam CD137 (4B4-1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD25 (MEM-181) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).
1A-143_FC_Histogram
Separation of cells stained using anti-human CD137 (4B4-1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1 μg/ml, same as CD137 APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cell suspension.

Product specific references:

Bellarosa D, Bressan A, Bigioni M, Parlani M, Maggi CA, Binaschi M: SAHA/Vorinostat induces the expression of the CD137 receptor/ligand system and enhances apoptosis mediated by soluble CD137 receptor in a human breast cancer cell line. Int J Oncol. 2012 Oct;41(4):1486-94.
PubMed
Fernández Do Porto DA, Jurado JO, Pasquinelli V, Alvarez IB, Aspera RH, Musella RM, García VE: CD137 differentially regulates innate and adaptive immunity against Mycobacterium tuberculosis. Immunol Cell Biol. 2012 Apr;90(4):449-56.
PubMed
Yi L, Zhao Y, Wang X, Dai M, Hellström KE, Hellström I, Zhang H: Human and mouse CD137 have predominantly different binding CRDs to their respective ligands. PLoS One. 2014 Jan 21;9(1):e86337.
PubMed
Chan SL, Voskens CJ, Lin W, Schindler DG, Azimzadeh A, Wang LX, Taylor RJ, Strome SE, Schulze DH: Epitope mapping of a chimeric CD137 mAb: a necessary step for assessing the biologic relevance of non-human primate models. J Mol Recognit. 2009 May-Jun;22(3):242-9. doi: 10.1002/jmr.937.
PubMed
Kienzle G, von Kempis J: CD137 (ILA/4-1BB), expressed by primary human monocytes, induces monocyte activation and apoptosis of B lymphocytes. Int Immunol. 2000 Jan;12(1):73-82.
PubMed
Salih HR, Kosowski SG, Haluska VF, Starling GC, Loo DT, Lee F, Aruffo AA, Trail PA, Kiener PA: Constitutive expression of functional 4-1BB (CD137) ligand on carcinoma cells. J Immunol. 2000 Sep 1;165(5):2903-10.
PubMed
Variant
100 tests
1A-143-T100
In stock
242.00 USD
Variant
100 tests
1A-143-T100
In stock
242.00 USD

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