Anti-Hu CD133 Purified

Anti-Hu CD133 Purified
Regulatory status
RUO
Antigen
CD133
Clone
W6B3C1
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-819-C100
In stock
187.00 USD
Variant
0.1 mg
11-819-C100
In stock
187.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody W6B3C1 recognizes the extracellular glycosylated epitope 1 on human CD133 (CD133/1), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of stem cells and tumor cells.
Workshop
HLDA VII Workshop
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions preferred.
Reactivity
Human
Immunogen
WERI-RB-1 retinoblastoma cell line
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Prominin 1, PROM1, AC133, PROML1, STGD4
Antigen description
CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.
Entrez Gene ID 8842
UniProt ID O43490
11-819 FC
Flow cytometry analysis (surface staining) of human peripheral whole blood showing CD133 positive stem cells (blue) and lymphocytes (red) stained using anti-CD133 (W6B3C1) purified, GAM-FITC.
11-819_WB
Western blotting analysis of human CD133 using mouse monoclonal antibody W6B3C1 on lysates of HT29 cells and Jurkat cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD133 was detected around 125 kDa.

Product specific references:

Liu C, Li Y, Xing Y, Cao B, Yang F, Yang T, Ai Z, Wei Y, Jiang J: The Interaction between Cancer Stem Cell Marker CD133 and Src Protein Promotes Focal Adhesion Kinase (FAK) Phosphorylation and Cell Migration. J Biol Chem. 2016 Jul 22;291(30):15540-50.
PubMed
Rappa G, Fodstad O, Lorico A: The stem cell-associated antigen CD133 (Prominin-1) is a molecular therapeutic target for metastatic melanoma. Stem Cells. 2008 Dec;26(12):3008-17.
PubMed
Hermansen SK, Christensen KG, Jensen SS, Kristensen BW: Inconsistent immunohistochemical expression patterns of four different CD133 antibody clones in glioblastoma. J Histochem Cytochem. 2011 Apr;59(4):391-407.
PubMed
Feng HL, Liu YQ, Yang LJ, Bian XC, Yang ZL, Gu B, Zhang H, Wang CJ, Su XL, Zhao XM: Expression of CD133 correlates with differentiation of human colon cancer cells. Cancer Biol Ther. 2010 Feb;9(3):216-23.
PubMed
Dahlrot RH, Hermansen SK, Hansen S, Kristensen BW: What is the clinical value of cancer stem cell markers in gliomas? Int J Clin Exp Pathol. 2013;6(3):334-48.
PubMed
Brescia P, Ortensi B, Fornasari L, Levi D, Broggi G, Pelicci G: CD133 is essential for glioblastoma stem cell maintenance. Stem Cells. 2013 May;31(5):857-69.
PubMed
Variant
0.1 mg
11-819-C100
In stock
187.00 USD
Variant
0.1 mg
11-819-C100
In stock
187.00 USD

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