Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD133 APC

Anti-Hu CD133 APC

Regulatory status

RUO

Antigen

CD133

Clone

W6B3C1

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-819-T100

In stock

242.00 USD

Variant

100 tests

1A-819-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody W6B3C1 recognizes the extracellular glycosylated epitope 1 on human CD133 (CD133/1), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of stem cells and tumor cells.

Workshop

HLDA VII Workshop

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

WERI-RB-1 retinoblastoma cell line

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Prominin 1, PROM1, AC133, PROML1, STGD4

Antigen description

CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.

Entrez Gene ID 8842

UniProt ID O43490

Flow cytometry surface staining pattern of human bone marrow cells stained using anti-human CD133 CD133 (W6B3C1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Flow cytometry multicolor surface staining of human bone marrow cells stained using anti-human CD133 (W6B3C1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension) and anti-human CD34 (581) PE antibody (20 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human CD133 positive CD34 positive stem cells (red-filled) from CD133 negative CD34 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human bone marrow cells stained using anti-human CD133 (W6B3C1) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Liu C, Li Y, Xing Y, Cao B, Yang F, Yang T, Ai Z, Wei Y, Jiang J: The Interaction between Cancer Stem Cell Marker CD133 and Src Protein Promotes Focal Adhesion Kinase (FAK) Phosphorylation and Cell Migration. J Biol Chem. 2016 Jul 22;291(30):15540-50.
PubMed

Rappa G, Fodstad O, Lorico A: The stem cell-associated antigen CD133 (Prominin-1) is a molecular therapeutic target for metastatic melanoma. Stem Cells. 2008 Dec;26(12):3008-17.
PubMed

Hermansen SK, Christensen KG, Jensen SS, Kristensen BW: Inconsistent immunohistochemical expression patterns of four different CD133 antibody clones in glioblastoma. J Histochem Cytochem. 2011 Apr;59(4):391-407.
PubMed

Feng HL, Liu YQ, Yang LJ, Bian XC, Yang ZL, Gu B, Zhang H, Wang CJ, Su XL, Zhao XM: Expression of CD133 correlates with differentiation of human colon cancer cells. Cancer Biol Ther. 2010 Feb;9(3):216-23.
PubMed

Dahlrot RH, Hermansen SK, Hansen S, Kristensen BW: What is the clinical value of cancer stem cell markers in gliomas? Int J Clin Exp Pathol. 2013;6(3):334-48.
PubMed

Brescia P, Ortensi B, Fornasari L, Levi D, Broggi G, Pelicci G: CD133 is essential for glioblastoma stem cell maintenance. Stem Cells. 2013 May;31(5):857-69.
PubMed