Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD132 APC

Anti-Hu CD132 APC

Regulatory status

RUO

Antigen

CD132

Clone

TUGh4

Format

APC

Reactivity

Human, Dog

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-194-T100

In stock

248.60 USD

Variant

100 tests

1A-194-T100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2b kappa

Specificity

The rat monoclonal antibody TUGh4 recognizes an extracellular epitope of CD132 (the common gamma chain), a 65-70 kDa type I transmembrane glycoprotein broadly expressed by most leukocytes.

Workshop

HLDA VI: WS Code C-89

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human, Dog

Immunogen

Human CD132-transfected cell line

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

common gamma chain, IL2RG, SCIDX, IMD4

Antigen description

CD132 / common gamma chain is an essential component of receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21, and it is critical for development of the immune system. Its mutation causes X-linked severe combined immunodeficiency disease (XSCID). CD132 is expressed on lymphocytes, NK cells, monocytes, and granulocytes. Through its cytoplasmic part which containsfour tyrosines and an SH2 domain, CD132 transcuces signal to downstream JAK/STAT pathway.

Entrez Gene ID 3561

UniProt ID P31785

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD132 (TUGh4) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD132 (TUGh4) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD132 (TUGh4) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Corrigall VM, Arastu M, Khan S, Shah C, Fife M, Smeets T, Tak PP, Panayi GS: Functional IL-2 receptor beta (CD122) and gamma (CD132) chains are expressed by fibroblast-like synoviocytes: activation by IL-2 stimulates monocyte chemoattractant protein-1 production. J Immunol. 2001 Mar 15;166(6):4141-7.
PubMed

Brisslert M, Bokarewa M, Larsson P, Wing K, Collins LV, Tarkowski A: Phenotypic and functional characterization of human CD25+ B cells. Immunology. 2006 Apr;117(4):548-57.
PubMed

Amu S, Brisslert M: Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood. Clin Dev Immunol. 2011;2011:481948.
PubMed

Fiorito S, Magrini L, Adrey J, Mailhé D, Brouty-Boyé D: Inflammatory status and cartilage regenerative potential of synovial fibroblasts from patients with osteoarthritis and chondropathy. Rheumatology (Oxford). 2005 Feb;44(2):164-71.
PubMed

Marino JH, Tan C, Taylor AA, Bentley C, Van De Wiele CJ, Ranne R, Paliotta M, Broughan TA, Teague TK: Differential IL-7 responses in developing human thymocytes. Hum Immunol. 2010 Apr;71(4):329-33.
PubMed

Itano M, Tsuchiya S, Morita S, Fujie H, Ishii N, Yanagisawa T, Ohashi Y, Minegishi M, Sugamura K, Konno T: IL-2 receptor gamma chain expression on CD34 positive hematopoietic progenitor cells from bone marrow and cord blood. Tohoku J Exp Med. 1996 Apr;178(4):389-98.
PubMed