Anti-Hu CD13 Purified

Anti-Hu CD13 Purified
Regulatory status
RUO
Antigen
CD13
Clone
WM15
Format
Purified
Reactivity
Human, Non-human primates
Variant
0.1 mg
11-396-C100
In stock
154.00 USD

0.025 mg
11-396-C025
Delivery 1 week
77.00 USD
Variant
0.1 mg
11-396-C100
In stock
154.00 USD

0.025 mg
11-396-C025
Delivery 1 week
77.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody WM15 recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.
Workshop
HLDA III: WS Code M 213; HLDA IV: WS Code M 44; HLDA IV: WS Code M 209; HLDA V: WS Code M MA191
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human, Non-human primates
Immunogen
Human AML cells
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Aminopeptidase N, APN, PEPN, ANPEP, gp150, LAP1
Antigen description
CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.
Entrez Gene ID 290
UniProt ID P15144
11-396_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD13 (WM15) purified antibody (concentration in sample 1 μg/ml, GAM APC).
11-396_FC_Histogram
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD13 (WM15) purified antibody (concentration in sample 1 μg/ml, GAM APC).

General references:

Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M. Clinical significance of aminopeptidase N in non-small cell lung cancer. Clin Cancer Res. 2006 Jul 1;12(13):3971-8.
PubMed
Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH. CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1;110(1):142-50.
PubMed
Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 27;7:140.
PubMed

Product specific references:

Favaloro EJ, Browning T, Facey D: CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol. 1993 Dec;21(13):1695-701.
PubMed
McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555.
PubMed
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987).
Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep;61(1):11-20.
PubMed
Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul;17(3):392-9.
PubMed
Variant
0.1 mg
11-396-C100
In stock
154.00 USD

0.025 mg
11-396-C025
Delivery 1 week
77.00 USD
Variant
0.1 mg
11-396-C100
In stock
154.00 USD

0.025 mg
11-396-C025
Delivery 1 week
77.00 USD

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