Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD13 Biotin

Anti-Hu CD13 Biotin

Regulatory status

RUO

Antigen

CD13

Clone

WM15

Format

Biotin

Reactivity

Human, Non-human primates

Application

IP, FC (QC tested), IHC(F)

Variant

0.1 mg

1B-396-C100

In stock

180.00 USD

0.025 mg

1B-396-C025

Delivery 1 week

90.00 USD

Variant

0.1 mg

1B-396-C100

In stock

180.00 USD

0.025 mg

1B-396-C025

Delivery 1 week

90.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody WM15 recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.

Workshop

HLDA III: WS Code M 213; HLDA IV: WS Code M 44; HLDA IV: WS Code M 209; HLDA V: WS Code M MA191

Application

IP, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 3-5 μg/ml.

Reactivity

Human, Non-human primates

Immunogen

Human AML cells

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Aminopeptidase N, APN, PEPN, ANPEP, gp150, LAP1

Antigen description

CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.

Entrez Gene ID 290

UniProt ID P15144

Flow cytometry analysis (surface staining) of human peripheral blood leukocytes with anti-CD13 (WM15) biotin, streptavidin-APC.

General references:

Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M. Clinical significance of aminopeptidase N in non-small cell lung cancer. Clin Cancer Res. 2006 Jul 1;12(13):3971-8.
PubMed

Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH. CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1;110(1):142-50.
PubMed

Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 27;7:140.
PubMed

Product specific references:

Favaloro EJ, Browning T, Facey D: CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol. 1993 Dec;21(13):1695-701.
PubMed

McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555.
PubMed

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987).

Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep;61(1):11-20.
PubMed

Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul;17(3):392-9.
PubMed