Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD129 PE

Anti-Hu CD129 PE

Regulatory status

RUO

Antigen

CD129

Clone

AH9R7

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-788-T100

In stock

242.00 USD

25 tests

1P-788-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-788-T100

In stock

242.00 USD

25 tests

1P-788-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody AH9R7 recognizes an extracellular epitope of CD129 / IL-9R alpha, a 57 kDa type I transmembrane glycoprotein expressed at low levels by lymphocytes, blood cell progenitors, eosinophils, mast cells, epithelial cells, muscle cells and neurons.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human CD129-transfected cell line

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IL9R

Antigen description

CD129 serves as the high affinity alpha subunit of IL-9 receptor. It associates with CD132, the common gamma chain shared by receptors of many different cytokines. CD129 is expressed at low levels by T and B cells, blood cell progenitors, eosinophils, mast cells, epithelial cells, muscle cells and neurons. Its signaling (through JAK/STAT pathways) results in proliferative and anti-apoptotic response, which is critical e.g. for intrathymic T cell development and survival of various cell types. The gene for CD129 is located at the pseudoautosomal regions of X and Y chromosomes and it may be related with the development of asthma.

Entrez Gene ID 3581

UniProt ID Q01113

Separation of HUT-78 cells stained using anti-human CD129 (AH9R7) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HUT-78 cells stained using mouse IgG2b isotype control (MPC-11) PE antibody (concentration in sample 5 μg/ml, same as CD129 PE antibody concentration, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

De Smedt M, Verhasselt B, Kerre T, Vanhecke D, Naessens E, Leclercq G, Renauld JC, Van Snick J, Plum J: Signals from the IL-9 receptor are critical for the early stages of human intrathymic T cell development. J Immunol. 2000 Feb 15;164(4):1761-7.
PubMed

Grasso L, Huang M, Sullivan CD, Messler CJ, Kiser MB, Dragwa CR, Holroyd KJ, Renauld JC, Levitt RC, Nicolaides NC: Molecular analysis of human interleukin-9 receptor transcripts in peripheral blood mononuclear cells. Identification of a splice variant encoding for a nonfunctional cell surface receptor. J Biol Chem. 1998 Sep 11;273(37):24016-24.
PubMed

Pilette C, Ouadrhiri Y, Van Snick J, Renauld JC, Staquet P, Vaerman JP, Sibille Y: IL-9 inhibits oxidative burst and TNF-alpha release in lipopolysaccharide-stimulated human monocytes through TGF-beta. J Immunol. 2002 Apr 15;168(8):4103-11.
PubMed

Pilette C, Ouadrhiri Y, Van Snick J, Renauld JC, Staquet P, Vaerman JP, Sibille Y: Oxidative burst in lipopolysaccharide-activated human alveolar macrophages is inhibited by interleukin-9. Eur Respir J. 2002 Nov;20(5):1198-205.
PubMed