Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD127 Purified

Anti-Hu CD127 Purified

Regulatory status

RUO

Antigen

CD127

Clone

rHIQB010

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-962-C100

In stock

158.40 USD

Variant

0.1 mg

11-962-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The recombinant mouse monoclonal antibody rHIQB010 recognizes an extracellular epitope of human CD127, the alpha subunit of IL-7 receptor.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 0.5-4 μg/ml.

Reactivity

Human

Description

Recombinant monoclonal antibody is expressed in CHO cell culture.

Preparation

Purified by affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IL7R, IMD104

Antigen description

CD127, a 60-90 kDa type I transmembrane glycoprotein, serves as the alpha subunit of the IL-7 receptor. It heterodimerizes with CD132, which is the common interleukin receptor gamma chain. CD127-mediated signaling promotes cell survival and is essential for lymphocyte development, including V(D)J recombination. Defects in CD127 can cause severe combined immunodeficiency (SCID). It is expressed in common lymphoid progenitors, B and T cell precursors (except CD4/CD8 double positive thymocytes), and mature T cell subpopulations. Majority of CD127-CD4+ T cells are Treg cells.

Entrez Gene ID 3575

UniProt ID P16871

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD127 (rHIQB010) purified antibody (concentration in sample 0.56 μg/ml, GAM APC).

Separation of human CD127 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD127 (rHIQB010) purified antibody (concentration in sample 0.56 μg/ml, GAM APC).