Anti-Hu CD127 Alexa Fluor® 647

Anti-Hu CD127 Alexa Fluor<sup>®</sup> 647
Regulatory status
RUO
Antigen
CD127
Clone
rHIQB010
Format
Alexa Fluor® 647
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
A6-962-T100
In stock
248.60 USD
Variant
100 tests
A6-962-T100
In stock
248.60 USD
Product details
Description
Images
SDS download
Isotype
Mouse IgG1
Specificity
The recombinant mouse monoclonal antibody rHIQB010 recognizes an extracellular epitope of human CD127, the alpha subunit of IL-7 receptor.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Preparation
Purified antibody is conjugated with Alexa Fluor® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
IL7R, IMD104
Antigen description
CD127, a 60-90 kDa type I transmembrane glycoprotein, serves as the alpha subunit of the IL-7 receptor. It heterodimerizes with CD132, which is the common interleukin receptor gamma chain. CD127-mediated signaling promotes cell survival and is essential for lymphocyte development, including V(D)J recombination. Defects in CD127 can cause severe combined immunodeficiency (SCID). It is expressed in common lymphoid progenitors, B and T cell precursors (except CD4/CD8 double positive thymocytes), and mature T cell subpopulations. Majority of CD127-CD4+ T cells are Treg cells.
Entrez Gene ID 3575
UniProt ID P16871
A6-962_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD127 (rHIQB010) Alexa Fluor® 647 antibody (concentration in sample 0.19 μg/ml).
A6-962_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD4 (MEM-241) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD127 (rHIQB010) Alexa Fluor® 647 antibody (concentration in sample 0.19 μg/ml).
A6-962_FC_Dot-plot_2
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD25 (MEM-181) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD127 (rHIQB010) Alexa Fluor® 647 antibody (concentration in sample 0.19 μg/ml).
A6-962_FC_Histogram
Separation of human CD127 positive lymphocytes (red-filled) from CD127 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD127 (rHIQB010) Alexa Fluor® 647 antibody (concentration in sample 0.19 μg/ml).
Variant
100 tests
A6-962-T100
In stock
248.60 USD
Variant
100 tests
A6-962-T100
In stock
248.60 USD

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