Anti-Hu CD126 Purified

Anti-Hu CD126 Purified
Regulatory status
RUO
Antigen
CD126
Clone
UV4
Format
Purified
Reactivity
Non-human primates, Human
Variant
0.1 mg
11-872-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody UV4 recognizes an extracellular epitope of CD126, a transmembrane glycoprotein serving as IL-6 receptor.
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Non-human primates, Human
Immunogen
human myeloma U266 cells
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
IL6RA, GP80, IL6Q
Antigen description
CD126 is a type I plasma membrane glycoprotein of the hematopoietin receptor superfamily. It binds IL-6 and then associates with CD130 homodimers to form the functional IL-6 receptor. CD126 alone binds IL-6 with low affinity, and after association with CD130 with high affinity. It is expressed on normal B cells, T cells, monocytes, and hepatocytes, as well as on neoplastic (but not on normal) plasma cells. It is being used as an endothelial marker for separation of circulating endothelial cells from blood.
Entrez Gene ID 3570
UniProt ID P08887
11-872_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD126 (UV4) purified antibody (concentration in sample 5 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
11-872_FC_Histogram
Separation of human CD126 positive lymphocytes (red-filled) from CD126 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD126 (UV4) purified antibody (concentration in sample 5 μg/ml, GAM APC.

Product specific references:

Lu L, Lan Q, Li Z, Zhou X, Gu J, Li Q, Wang J, Chen M, Liu Y, Shen Y, Brand DD, Ryffel B, Horwitz DA, Quismorio FP, Liu Z, Li B, Olsen NJ, Zheng SG: Critical role of all-trans retinoic acid in stabilizing human natural regulatory T cells under inflammatory conditions. Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):E3432-40.
PubMed
Zegeye MM, Lindkvist M, Fälker K, Kumawat AK, Paramel G, Grenegård M, Sirsjö A, Ljungberg LU: Activation of the JAK/STAT3 and PI3K/AKT pathways are crucial for IL-6 trans-signaling-mediated pro-inflammatory response in human vascular endothelial cells. Cell Commun Signal. 2018 Sep 5;16(1):55.
PubMed
Hashwah H, Bertram K, Stirm K, Stelling A, Wu CT, Kasser S, Manz MG, Theocharides AP, Tzankov A, Müller A: The IL-6 signaling complex is a critical driver, negative prognostic factor, and therapeutic target in diffuse large B-cell lymphoma. EMBO Mol Med. 2019 Oct;11(10):e10576.
PubMed
Laudanski K, Qing M, Oszkiel H, Zawadka M, Lapko N, Nowak Z, Worthen GS: Ketamine affects in vitro differentiation of monocyte into immature dendritic cells. Anesthesiology. 2015 Sep;123(3):628-41.
PubMed
Couturier J, Suliburk JW, Brown JM, Luke DJ, Agarwal N, Yu X, Nguyen C, Iyer D, Kozinetz CA, Overbeek PA, Metzker ML, Balasubramanyam A, Lewis DE: Human adipose tissue as a reservoir for memory CD4+ T cells and HIV. AIDS. 2015 Mar 27;29(6):667-74.
PubMed
Huang YW, Vitetta ES: A monoclonal anti-human IL-6 receptor antibody inhibits the proliferation of human myeloma cells. Hybridoma. 1993 Oct;12(5):621-30.
PubMed
Schumacher N, Meyer D, Mauermann A, von der Heyde J, Wolf J, Schwarz J, Knittler K, Murphy G, Michalek M, Garbers C, Bartsch JW, Guo S, Schacher B, Eickholz P, Chalaris A, Rose-John S, Rabe B: Shedding of endogenous interleukin-6 receptor (IL-6R) is governed by a disintegrin and metalloproteinase (ADAM) proteases while a full-length IL-6R isoform localizes to circulating microvesicles. J Biol Chem. 2015 Oct 23;290(43):26059-71.
PubMed
Variant
0.1 mg
11-872-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

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