Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD126 Purified

Anti-Hu CD126 Purified

Regulatory status

RUO

Antigen

CD126

Clone

UV4

Format

Purified

Reactivity

Non-human primates, Human

Application

IP, WB, FC (QC tested)

Variant

0.1 mg

11-872-C100

In stock

154.00 USD

Variant

0.1 mg

11-872-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody UV4 recognizes an extracellular epitope of CD126, a transmembrane glycoprotein serving as IL-6 receptor.

Application

IP, WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2-5 μg/ml.

Reactivity

Non-human primates, Human

Immunogen

human myeloma U266 cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IL6RA, GP80, IL6Q

Antigen description

CD126 is a type I plasma membrane glycoprotein of the hematopoietin receptor superfamily. It binds IL-6 and then associates with CD130 homodimers to form the functional IL-6 receptor. CD126 alone binds IL-6 with low affinity, and after association with CD130 with high affinity. It is expressed on normal B cells, T cells, monocytes, and hepatocytes, as well as on neoplastic (but not on normal) plasma cells. It is being used as an endothelial marker for separation of circulating endothelial cells from blood.

Entrez Gene ID 3570

UniProt ID P08887

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD126 (UV4) purified antibody (concentration in sample 5 μg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD126 positive lymphocytes (red-filled) from CD126 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD126 (UV4) purified antibody (concentration in sample 5 μg/ml, GAM APC.

Product specific references:

Lu L, Lan Q, Li Z, Zhou X, Gu J, Li Q, Wang J, Chen M, Liu Y, Shen Y, Brand DD, Ryffel B, Horwitz DA, Quismorio FP, Liu Z, Li B, Olsen NJ, Zheng SG: Critical role of all-trans retinoic acid in stabilizing human natural regulatory T cells under inflammatory conditions. Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):E3432-40.
PubMed

Zegeye MM, Lindkvist M, Fälker K, Kumawat AK, Paramel G, Grenegård M, Sirsjö A, Ljungberg LU: Activation of the JAK/STAT3 and PI3K/AKT pathways are crucial for IL-6 trans-signaling-mediated pro-inflammatory response in human vascular endothelial cells. Cell Commun Signal. 2018 Sep 5;16(1):55.
PubMed

Hashwah H, Bertram K, Stirm K, Stelling A, Wu CT, Kasser S, Manz MG, Theocharides AP, Tzankov A, Müller A: The IL-6 signaling complex is a critical driver, negative prognostic factor, and therapeutic target in diffuse large B-cell lymphoma. EMBO Mol Med. 2019 Oct;11(10):e10576.
PubMed

Laudanski K, Qing M, Oszkiel H, Zawadka M, Lapko N, Nowak Z, Worthen GS: Ketamine affects in vitro differentiation of monocyte into immature dendritic cells. Anesthesiology. 2015 Sep;123(3):628-41.
PubMed

Couturier J, Suliburk JW, Brown JM, Luke DJ, Agarwal N, Yu X, Nguyen C, Iyer D, Kozinetz CA, Overbeek PA, Metzker ML, Balasubramanyam A, Lewis DE: Human adipose tissue as a reservoir for memory CD4+ T cells and HIV. AIDS. 2015 Mar 27;29(6):667-74.
PubMed

Huang YW, Vitetta ES: A monoclonal anti-human IL-6 receptor antibody inhibits the proliferation of human myeloma cells. Hybridoma. 1993 Oct;12(5):621-30.
PubMed

Schumacher N, Meyer D, Mauermann A, von der Heyde J, Wolf J, Schwarz J, Knittler K, Murphy G, Michalek M, Garbers C, Bartsch JW, Guo S, Schacher B, Eickholz P, Chalaris A, Rose-John S, Rabe B: Shedding of endogenous interleukin-6 receptor (IL-6R) is governed by a disintegrin and metalloproteinase (ADAM) proteases while a full-length IL-6R isoform localizes to circulating microvesicles. J Biol Chem. 2015 Oct 23;290(43):26059-71.
PubMed