Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD123 Purified

Anti-Hu CD123 Purified

Regulatory status

RUO

Antigen

CD123

Clone

6H6

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC(F)

Variant

0.1 mg

11-700-C100

In stock

143.00 USD

0.025 mg

11-700-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-700-C100

In stock

143.00 USD

0.025 mg

11-700-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.

Application

IP, WB, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IL3RA, MGC34174, hIL-3Ra

Antigen description

CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.

Entrez Gene ID 3563

UniProt ID P26951

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) purified antibody (concentration in sample 0,11 μg/ml, GAM APC).

Separation of CD123 positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD123 (6H6) purified antibody (concentration in sample 0,11 μg/ml, GAM APC).

Product specific references:

Sun Q, Woodcock JM, Rapoport A, Stomski FC, Korpelainen EI, Bagley CJ, Goodall GJ, Smith WB, Gamble JR, Vadas MA, Lopez AF: Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist. Blood. 1996 Jan 1;87(1):83-92.
PubMed

Martín-Gayo E, Sierra-Filardi E, Corbí AL, Toribio ML: Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development. Blood. 2010 Jul 1;115(26):5366-75.
PubMed

Herling M, Teitell MA, Shen RR, Medeiros LJ, Jones D: TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin. Blood. 2003 Jun 15;101(12):5007-9.
PubMed