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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD123 PE
Anti-Hu CD123 PE
Regulatory status
RUO
Antigen
CD123
Clone
6H6
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-700-T100
In stock
220.00 USD
25 tests
1P-700-T025
In stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
0.1 mg
11-261-C100
In stock
110.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
Other names
IL3RA, MGC34174, hIL-3Ra
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD123 (6H6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD123 positive Basophil granulocytes(red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Product specific references:
Sun Q, Woodcock JM, Rapoport A, Stomski FC, Korpelainen EI, Bagley CJ, Goodall GJ, Smith WB, Gamble JR, Vadas MA, Lopez AF: Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist. Blood. 1996 Jan 1;87(1):83-92.
PubMed
Martín-Gayo E, Sierra-Filardi E, Corbí AL, Toribio ML: Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development. Blood. 2010 Jul 1;115(26):5366-75.
PubMed
Herling M, Teitell MA, Shen RR, Medeiros LJ, Jones D: TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin. Blood. 2003 Jun 15;101(12):5007-9.
PubMed
Variant
100 tests
1P-700-T100
In stock
220.00 USD
25 tests
1P-700-T025
In stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
0.1 mg
11-261-C100
In stock
110.00 USD
Contact distributor
Datasheet download
SDS download
CS
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Related products
PB-222-T100
Anti-Hu CD45 Pacific Blue™
-134%
Clone
MEM-28
Reg. status
RUO
Application
FC
100 tests
220.00 USD
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