Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD122 Purified Low Endotoxin

Anti-Hu CD122 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD122

Clone

TU27

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, FC (QC tested), FUNC

Variant

0.1 mg

12-195-C100

Out of stock

130.00 USD

Variant

0.1 mg

11-261-C100

In stock

130.00 USD

Contact distributor

Product details

Images

References

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody TU27 recognizes an extracellular epitope of CD122 (IL-2R beta), a 70-75 kDa type I transmembrane glycoprotein constitutively expressed by NK cells and a T cell subset, and upregulated upon activation.

Workshop

HLDA V: WS Code C050

Application

IP, FC (QC tested), FUNC

Application details

Functional application: Blocking.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

TL-Mor cell line

Other names

IL2RB, IL15RB, P70-75

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography

Formulation

Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.

Storage and handling

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD122 (TU27) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Flow cytometry multicolor surface staining of human CD3 negative lymphocytes stained using anti-human CD122 (TU27) purified antibody (low endotoxin, concentration in sample 4 μg/ml, GAM APC) and anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD122 positive CD56 positive CD3 negative NK cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD122 (TU27) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Product specific references:

Elkins J, Sheridan J, Amaravadi L, Riester K, Selmaj K, Bielekova B, Parr E, Giovannoni G: CD56(bright) natural killer cells and response to daclizumab HYP in relapsing-remitting MS. Neurol Neuroimmunol Neuroinflamm. 2015 Jan 22;2(2):e65.
PubMed

Gallart T, de la Fuente MA, Barceló JJ, Alberola-Ila J, Lozano F: Desialylation of T lymphocytes overcomes the monocyte dependency of pokeweed mitogen-induced T-cell activation. Immunology 1997 Jan;90(1):57-65.
PubMed

Sharkey AM, Xiong S, Kennedy PR, Gardner L, Farrell LE, Chazara O, Ivarsson MA, Hiby SE, Colucci F, Moffett A: Tissue-Specific Education of Decidual NK Cells. J Immunol. 2015 Oct 1;195(7):3026-32.
PubMed

Ozawa A, Tada H, Tamai R, Uehara A, Watanabe K, Yamaguchi T, Shimauchi H, Takada H, Sugawara S: Expression of IL-2 receptor beta and gamma chains by human gingival fibroblasts and up-regulation of adhesion to neutrophils in response to IL-2. J Leukoc Biol. 2003 Sep;74(3):352-9.
PubMed

Sahraoui Y, Perraki M, Theodoropoulou M, Allouche M, Tsapis A, Ammar A, Clemenceau C, Bokogiorgou S, Yacci T, Katrinakis G, Jasmin C, Georgoulias V: Autocrine IL-2-dependent growth of a newly established CD3+, CD16-, CD56+, CD57+, J(H)-, TCRbeta-, TCRgamma- leukemia cell line (NOI-90). Leukemia. 1997 Feb;11(2):245-52.
PubMed

Huss DJ, Mehta DS, Sharma A, You X, Riester KA, Sheridan JP, Amaravadi LS, Elkins JS, Fontenot JD: In vivo maintenance of human regulatory T cells during CD25 blockade. J Immunol. 2015 Jan 1;194(1):84-92.
PubMed

Variant

0.1 mg

12-195-C100

Out of stock

130.00 USD

Variant

0.1 mg

11-261-C100

In stock

130.00 USD

Contact distributor

Datasheet download