Anti-Hu CD122 PE

Anti-Hu CD122 PE
Regulatory status
RUO
Antigen
CD122
Clone
TU27
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-195-T100
In stock
242.00 USD

25 tests
1P-195-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-195-T100
In stock
242.00 USD

25 tests
1P-195-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody TU27 recognizes an extracellular epitope of CD122 (IL-2R beta), a 70-75 kDa type I transmembrane glycoprotein constitutively expressed by NK cells and a T cell subset, and upregulated upon activation.
Workshop
HLDA V: WS Code C050
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
TL-Mor cell line
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
IL2RB, IL15RB, P70-75
Antigen description
CD122 (IL-2/IL-15R beta) constitutes together with CD132 (common gamma chain) and with CD25 (IL-2/IL15R alpha) the intermediate (CD122+CD132) and the high affinity (CD122+CD132+CD25) IL-2 and IL-15 receptor complex. CD122 is expressed on NK cells and lymphocytes, but at low level, unless the cell is activated. The cytoplasmic part of CD122 binds to Src-family and Jak-family kinases. The biological effect of CD122 ligation depends on whether IL-2 or IL-15 is bound to the receptor complex.
Entrez Gene ID 3560
UniProt ID P14784
1P-195_FC_CDMaps_Profil značení
Anti-human CD122 PE antibody (clone TU27) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Mouse monoclonal anti-human CD122 PE antibody (clone TU27) was used in concentration 1 µg/ml in stained blood sample (2 x 106 cells).
1P-195_FC_CDMaps_Histogram adapt
Expression profiling on peripheral blood subsets using Anti-human CD122 PE antibody (clone TU27). Adaptive panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human CD122 PE antibody (clone TU27, 1 µg/ml in stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Adaptive) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.
1P-195_FC_CDMaps_Histogram innat
Expression profiling on peripheral blood subsets using Anti-human CD122 PE antibody (clone TU27). Innate panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human CD122 PE antibody (clone TU27, 1 µg/ml in stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Innate) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Product specific references:

Elkins J, Sheridan J, Amaravadi L, Riester K, Selmaj K, Bielekova B, Parr E, Giovannoni G: CD56(bright) natural killer cells and response to daclizumab HYP in relapsing-remitting MS. Neurol Neuroimmunol Neuroinflamm. 2015 Jan 22;2(2):e65.
PubMed
Gallart T, de la Fuente MA, Barceló JJ, Alberola-Ila J, Lozano F: Desialylation of T lymphocytes overcomes the monocyte dependency of pokeweed mitogen-induced T-cell activation. Immunology 1997 Jan;90(1):57-65.
PubMed
Sharkey AM, Xiong S, Kennedy PR, Gardner L, Farrell LE, Chazara O, Ivarsson MA, Hiby SE, Colucci F, Moffett A: Tissue-Specific Education of Decidual NK Cells. J Immunol. 2015 Oct 1;195(7):3026-32.
PubMed
Ozawa A, Tada H, Tamai R, Uehara A, Watanabe K, Yamaguchi T, Shimauchi H, Takada H, Sugawara S: Expression of IL-2 receptor beta and gamma chains by human gingival fibroblasts and up-regulation of adhesion to neutrophils in response to IL-2. J Leukoc Biol. 2003 Sep;74(3):352-9.
PubMed
Sahraoui Y, Perraki M, Theodoropoulou M, Allouche M, Tsapis A, Ammar A, Clemenceau C, Bokogiorgou S, Yacci T, Katrinakis G, Jasmin C, Georgoulias V: Autocrine IL-2-dependent growth of a newly established CD3+, CD16-, CD56+, CD57+, J(H)-, TCRbeta-, TCRgamma- leukemia cell line (NOI-90). Leukemia. 1997 Feb;11(2):245-52.
PubMed
Huss DJ, Mehta DS, Sharma A, You X, Riester KA, Sheridan JP, Amaravadi LS, Elkins JS, Fontenot JD: In vivo maintenance of human regulatory T cells during CD25 blockade. J Immunol. 2015 Jan 1;194(1):84-92.
PubMed
Variant
100 tests
1P-195-T100
In stock
242.00 USD

25 tests
1P-195-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-195-T100
In stock
242.00 USD

25 tests
1P-195-T025
Delivery 1 week
121.00 USD

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