Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD120a Purified

Anti-Hu CD120a Purified

Regulatory status

RUO

Antigen

CD120a

Clone

H398

Format

Purified

Reactivity

Human

Application

IP, FC (QC tested), IHC(F), IHC(P)

Variant

0.1 mg

11-773-C100

In stock

143.00 USD

0.025 mg

11-773-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-773-C100

In stock

143.00 USD

0.025 mg

11-773-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody H398 recognizes the extracellular domain of CD120a, a 55 kDa receptor for tumor necrosis factor. The antibody blocks biological activity of both natural and recombinant human TNF alpha and TNF beta.

Application

IP, FC (QC tested), IHC(F), IHC(P)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

Recombinant full length human CD120a

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TNF R1, TNFRSF1A, TNFR55, FPF, TBP1, p60

Antigen description

CD120a / TNF R1, also known as TNFR55 or TNFRSF1A, is a 55 kDa receptor for tumor necrosis factor alpha and it is expressed in most tissues. By binding its trimeric ligand the CD120a protein forms trimers and the conformation change leads to dissociation of the inhibitory factor SODD from its intracellular death domain and in formation of signaling platform. CD120a can mediate apoptosis, and function as a regulator of inflammation. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.

Entrez Gene ID 7132

UniProt ID P19438

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD120a (H398) purified antibody (concentration in sample 3 μg/ml) GAM APC.

Separation of human monocytes (red-filled) from CD120a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD120a (H398) purified antibody (concentration in sample 3 μg/ml) GAM APC.

Product specific references:

Buckley CD, Ross EA, McGettrick HM, Osborne CE, Haworth O, Schmutz C, Stone PC, Salmon M, Matharu NM, Vohra RK, Nash GB, Rainger GE: Identification of a phenotypically and functionally distinct population of long-lived neutrophils in a model of reverse endothelial migration. J Leukoc Biol. 2006 Feb;79(2):303-11.
PubMed

Kohrgruber N, Halanek N, Gröger M, Winter D, Rappersberger K, Schmitt-Egenolf M, Stingl G, Maurer D: Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines. J Immunol. 1999 Sep 15;163(6):3250-9.
PubMed

Baker PK, Pettitt AR, Slupsky JR, Chen HJ, Glenn MA, Zuzel M, Cawley JC: Response of hairy cells to IFN-alpha involves induction of apoptosis through autocrine TNF-alpha and protection by adhesion. Blood. 2002 Jul 15;100(2):647-53.
PubMed