Anti-Hu CD11b Purified Azide Free

Anti-Hu CD11b Purified Azide Free
Regulatory status
RUO
Antigen
CD11b
Clone
MEM-170
Format
Purified Azide Free
Reactivity
Human
Variant
0.1 mg
10-502-C100
In stock
130.00 USD

0.025 mg
10-502-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-170 recognizes an extracellular epitope of CD11b antigen (Mac-1alpha), a 165-170 kDa transmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells. The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
Workshop
HLDA VI: WS Code A054
Application details
Functional application: The antibody MEM-170 blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
Flow cytometry: Recommended dilution: 1 μg/ml.
Reactivity
Human
Immunogen
Human granulocytes
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 μm filter sterilized
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A
Antigen description
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Entrez Gene ID 3684
UniProt ID P11215
10-502 FC
Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD11b (MEM-170) purified, GAM-APC.

General references:

Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20.
PubMed
Lawrence PK, Srikumaran S: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98.
PubMed
Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606.
PubMed

Product specific references:

Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38.
PubMed
Variant
0.1 mg
10-502-C100
In stock
130.00 USD

0.025 mg
10-502-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD