Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD11b Purified Azide Free

Anti-Hu CD11b Purified Azide Free

Regulatory status

RUO

Antigen

CD11b

Clone

MEM-170

Format

Purified Azide Free

Reactivity

Human

Application

FC (QC tested), FUNC

Variant

0.1 mg

10-502-C100

In stock

143.00 USD

0.025 mg

10-502-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

10-502-C100

In stock

143.00 USD

0.025 mg

10-502-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-170 recognizes an extracellular epitope of CD11b antigen (Mac-1alpha), a 165-170 kDa transmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells. The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.

Workshop

HLDA VI: WS Code A054

Application

FC (QC tested), FUNC

Application details

Functional application: The antibody MEM-170 blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
Flow cytometry: Recommended dilution: 1 μg/ml.

Reactivity

Human

Immunogen

Human granulocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

Antigen description

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

Entrez Gene ID 3684

UniProt ID P11215

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD11b (MEM-170) purified, GAM-APC.

General references:

Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20.
PubMed

Lawrence PK, Srikumaran S: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98.
PubMed

Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606.
PubMed

Product specific references:

Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38.
PubMed