Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD11b APC

Anti-Hu CD11b APC

Regulatory status

RUO

Antigen

CD11b

Clone

MEM-174

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-211-T100

In stock

242.00 USD

25 tests

1A-211-T025

In stock

121.00 USD

Variant

100 tests

1A-211-T100

In stock

242.00 USD

25 tests

1A-211-T025

In stock

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

Workshop

HLDA VI: WS Code BP 310; HLDA VI: WS Code M 18

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human granulocytes

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

Antigen description

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

Entrez Gene ID 3684

UniProt ID P11215

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD11b (MEM-174) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human monocytes (red-filled) from CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11b (MEM-174) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

General references:

Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20.
PubMed

Lawrence PK, Srikumaran S: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98.
PubMed

Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606.
PubMed

Product specific references:

Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
PubMed

Hasan S, Osickova A, Bumba L, Novák P, Sebo P, Osicka R: Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding. FEBS Lett. 2015 Jan 30;589(3):374-9.
PubMed