Anti-Hu CD11b (activation epitope) Purified Low Endotoxin

Anti-Hu CD11b (activation epitope) Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD11b (activation epitope)
Clone
CBRM1/5
Format
Purified Low Endotoxin
Reactivity
Human
Variant
0.1 mg
12-172-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody CBRM1/5 recognizes an activation-dependent epitope on extracellular part of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol esters and it does not recognize CD11b on non-activated cells.
Application details
Functional application: Blocking.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
Information not available
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A
Antigen description
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Entrez Gene ID 3684
UniProt ID P11215
12-172_FC_Profil Značení
Flow cytometry surface staining pattern of human PMA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD11b activation epitope (CBRM1/5) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.
12-172_FC_Histogram
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PMA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD11b activation epitope(CBRM1/5) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Product specific references:

Fagerholm SC, Varis M, Stefanidakis M, Hilden TJ, Gahmberg CG: alpha-Chain phosphorylation of the human leukocyte CD11b/CD18 (Mac-1) integrin is pivotal for integrin activation to bind ICAMs and leukocyte extravasation. Blood. 2006 Nov 15;108(10):3379-86.
PubMed
Diamond MS, Springer TA: A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol. 1993 Jan;120(2):545-56.
PubMed
Variant
0.1 mg
12-172-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

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