Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD11b (activation epitope) Purified

Anti-Hu CD11b (activation epitope) Purified

Regulatory status

RUO

Antigen

CD11b (activation epitope)

Clone

CBRM1/5

Format

Purified

Reactivity

Human

Application

IP, FC (QC tested)

Variant

0.1 mg

11-172-C100

In stock

143.00 USD

0.025 mg

11-172-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-172-C100

In stock

143.00 USD

0.025 mg

11-172-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody CBRM1/5 recognizes an activation-dependent epitope on extracellular part of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol esters and it does not recognize CD11b on non-activated cells.

Application

IP, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

Information not available

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

Antigen description

CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.

Entrez Gene ID 3684

UniProt ID P11215

Flow cytometry surface staining pattern of human PMA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD11b activation epitope (CBRM1/5) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PMA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD11b activation epitope (CBRM1/5) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Product specific references:

Fagerholm SC, Varis M, Stefanidakis M, Hilden TJ, Gahmberg CG: alpha-Chain phosphorylation of the human leukocyte CD11b/CD18 (Mac-1) integrin is pivotal for integrin activation to bind ICAMs and leukocyte extravasation. Blood. 2006 Nov 15;108(10):3379-86.
PubMed

Diamond MS, Springer TA: A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol. 1993 Jan;120(2):545-56.
PubMed