Anti-Hu CD11b (activation epitope) Purified

Anti-Hu CD11b (activation epitope) Purified
Regulatory status
RUO
Antigen
CD11b (activation epitope)
Clone
CBRM1/5
Format
Purified
Reactivity
Human
Application
IP, FC (QC tested)
Variant
0.1 mg
11-172-C100
In stock
0.00 USD

0.025 mg
11-172-C025
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD

0.1 mg
11-261-C100
In stock
0.00 USD
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody CBRM1/5 recognizes an activation-dependent epitope on extracellular part of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol esters and it does not recognize CD11b on non-activated cells.
Application
IP, FC (QC tested)
Reactivity
Human
Immunogen
Information not available
Other names
Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-172_FC_Profil Značení
Flow cytometry surface staining pattern of human PMA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD11b activation epitope (CBRM1/5) purified antibody (concentration in sample 4 μg/ml) GAM APC.
11-172_FC_Histogram
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PMA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD11b activation epitope (CBRM1/5) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Product specific references:

Fagerholm SC, Varis M, Stefanidakis M, Hilden TJ, Gahmberg CG: alpha-Chain phosphorylation of the human leukocyte CD11b/CD18 (Mac-1) integrin is pivotal for integrin activation to bind ICAMs and leukocyte extravasation. Blood. 2006 Nov 15;108(10):3379-86.
PubMed
Diamond MS, Springer TA: A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol. 1993 Jan;120(2):545-56.
PubMed
Variant
0.1 mg
11-172-C100
In stock
0.00 USD

0.025 mg
11-172-C025
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD

0.1 mg
11-261-C100
In stock
0.00 USD