Anti-Hu CD11a Purified

Anti-Hu CD11a Purified
Regulatory status
RUO
Antigen
CD11a
Clone
MEM-83
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-380-C100
Ask for delivery term HERE
130.00 USD

0.025 mg
11-380-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-83 reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Workshop
HLDA IV: WS Code N 211
Application details
Flow cytometry: Recommended dilution: 1 μg/ml.
Reactivity
Human
Immunogen
Human peripheral blood lymphocytes
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LFA-1, LFA1A, ITGAL
Antigen description
CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Entrez Gene ID 3683
UniProt ID P20701
11-380_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD11a (MEM-83) purified antibody (concentration in sample 1 μg/ml) GAM APC.
11-380_FC_Histogram
Separation of human CD11a positive lymphocytes (red-filled) from CD11a negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11a (MEM-83) purified antibody (concentration in sample 1 μg/ml) GAM APC.

General references:

Sarantos MR, Raychaudhuri S, Lum AF, Staunton DE, Simon SI: Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1. J Biol Chem. 2005 Aug 5;280(31):28290-8.
PubMed
Giblin PA, Lemieux RM: LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics. Curr Pharm Des. 2006;12(22):2771-95.
PubMed
Kellersch B, Kolanus W: Membrane-proximal signaling events in beta-2 integrin activation. Results Probl Cell Differ. 2006;43:245-57.
PubMed
Fujii T, Takada-Takatori Y, Kawashima K: Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity. Life Sci. 2007 May 30;80(24-25):2320-4.
PubMed

Product specific references:

Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N: Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3. J Cell Biol. 1994 Jul;126(2):529-37.
PubMed
Landis RC, Bennett RI, Hogg N: A novel LFA-1 activation epitope maps to the I domain. J Cell Biol. 1993 Mar;120(6):1519-27.
PubMed
Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
Variant
0.1 mg
11-380-C100
Ask for delivery term HERE
130.00 USD

0.025 mg
11-380-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD