Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD119 Purified

Anti-Hu CD119 Purified

Regulatory status

RUO

Antigen

CD119

Clone

GIR-208

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested)

Variant

0.1 mg

11-875-C100

In stock

154.00 USD

Variant

0.1 mg

11-875-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody GIR-208 recognizes an epitope within extracellular part of CD119, a transmembrane glycoprotein of cytokine receptor family.

Workshop

HLDA VI: C-110

Application

IP, WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

CD119 purified from human placenta

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IFNGR1, IFNgammaRa, IMD27

Antigen description

CD119 (alpha subunit of interferon gamma receptor) is a type I transmembrane glycoprotein, which associates with IFNgammaAF1 to form functional receptor for interferon gamma, although CD119 alone is able to bind the cytokin. It is expressed on monocytes, macrophages, lymphocytes, NK cells, neutrophils, fibroblasts, epithelial and endothelial cells, as well as in many tumours.

Entrez Gene ID 3459

UniProt ID P15260

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD119 (GIR-208) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Separation of monocytes stained anti-human CD119 (GIR-208) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Fujii K, Miyahara Y, Harada N, Muraoka D, Komura M, Yamaguchi R, Yagita H, Nakamura J, Sugino S, Okumura S, Imoto S, Miyano S, Shiku H: Identification of an immunogenic neo-epitope encoded by murine sarcoma using CXCR3 ligand mRNAs as sensors. Oncoimmunology. 2017 Mar 20;6(5):e1306617.
PubMed

Alavi S, Stewart AJ, Kefford RF, Lim SY, Shklovskaya E, Rizos H: Interferon signaling is frequently downregulated in melanoma. Front Immunol. 2018 Jun 21;9:1414.
PubMed

Crescioli C, Cosmi L, Borgogni E, Santarlasci V, Gelmini S, Sottili M, Sarchielli E, Mazzinghi B, Francalanci M, Pezzatini A, Perigli G, Vannelli GB, Annunziato F, Serio M: Methimazole inhibits CXC chemokine ligand 10 secretion in human thyrocytes. Endocrinol. 2007 Oct;195(1):145-55.
PubMed

Igarashi T, Teramoto K, Ishida M, Hanaoka J, Daigo Y: The mechanism of de novo expression of programmed cell death-ligand 1 in squamous cell carcinoma of the lung. Oncol Rep. 2017 Oct;38(4):2189-2196.
PubMed

Hoh A, Heeg M, Ni Y, Schuch A, Binder B, Hennecke N, Blum HE, Nassal M, Protzer U, Hofmann M, Urban S, Thimme R: Hepatitis B virus-infected HepG2hNTCP cells serve as a novel immunological tool to analyze the antiviral efficacy of CD8+ T cells in vitro. J Virol. 2015 Jul;89(14):7433-8.
PubMed

El Darzi E, Bazzi S, Daoud S, Echtay KS, Bahr GM: Differential regulation of surface receptor expression, proliferation, and apoptosis in HaCaT cells stimulated with interferon-γ, interleukin-4, tumor necrosis factor-α, or muramyl dipeptide. Int J Immunopathol Pharmacol. 2017 Jun;30(2):130-145.
PubMed