Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD118 PE

Anti-Hu CD118 PE

Regulatory status

RUO

Antigen

CD118

Clone

12D3

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-916-C100

In stock

242.00 USD

Variant

0.1 mg

1P-916-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 12D3 recognizes an extracellular epitope of CD118, a transmembrane glycoprotein, which associates with CD130 to form the functional high affinity LIF receptor.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 4-10 μg/ml.

Reactivity

Human

Immunogen

human recombinant soluble CD118-Fc

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LIFR alpha, SWS, SJS2, STWSm, gp190

Antigen description

CD118 (LIFR alpha) is a type I transmembrane glycoprotein of the cytokine receptor family, which associates noncovalently with CD130 to form the functional high affinity LIF receptor, which also acts as an oncostatin M receptor. CD118 alone binds LIF with low affinity. A secreted form of CD118 results from alternative splicing, and may have inhibitory effect, as it also binds LIF, although with low affinity. CD118 is not expressed on lymphocytes, but it is widely expressed outside the immune system. Soluble CD118 level rises during pregnancy, in parallel with a drop in circulating LIF levels.

Entrez Gene ID 3977

UniProt ID P42702

Separation of JAR cells stained using anti-human CD118 (12D3) PE antibody (concentration in sample 15 μg/ml, red-filled) from JAR cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 15 μg/ml, same as CD118 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of JAR cell suspension.

Product specific references:

Taupin JL, Legembre P, Bitard J, Daburon S, Pitard V, Blanchard F, Duplomb L, Godard A, Jacques Y, Moreau JF: Identification of agonistic and antagonistic antibodies against gp190, the leukemia inhibitory factor receptor, reveals distinct roles for its two cytokine-binding domains. J Biol Chem. 2001 Dec 21;276(51):47975-81.
PubMed