Anti-Hu CD117 PE

Anti-Hu CD117 PE
Regulatory status
RUO
Antigen
CD117
Clone
104D2
Format
PE
Reactivity
Non-human primates, Human, Cow
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-586-T100
In stock
242.00 USD

25 tests
1P-586-T025
In stock
121.00 USD
Variant
100 tests
1P-586-T100
In stock
242.00 USD

25 tests
1P-586-T025
In stock
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.
Workshop
HLDA VI: WS Code C-30
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human, Cow
Immunogen
MOLM-1 megakaryocytic cells
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
c-Kit, PBT, SCFR
Antigen description
CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.
Entrez Gene ID 3815
UniProt ID P10721
1P-586 FC
Flow cytometry analysis (surface staining) of human peripheral blood cells showing CD117 positive cells (red) stained using anti-CD117 (104D2) PE.

Product specific references:

Stevenson KS, Mc Glynn L, Hodge M, Mc Linden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem Cells Dev. 2009 Mar 27.
PubMed
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007 Jul 1;110(1):151-60.
PubMed
Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk Res. 2006 Oct;30(10):1241-8.
PubMed
Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117). Exp Hematol. 2000 Jun;28(6):660-71.
PubMed
Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000 Apr;49(2):97-110.
PubMed
Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c-Kit. Blood. 1999 Sep 15;94(6):1979-86.
PubMed
Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer. Blood. 1998 Feb 1;91(3):898-906.
PubMed
Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997 Jul 1;90(1):111-25.
PubMed
Variant
100 tests
1P-586-T100
In stock
242.00 USD

25 tests
1P-586-T025
In stock
121.00 USD
Variant
100 tests
1P-586-T100
In stock
242.00 USD

25 tests
1P-586-T025
In stock
121.00 USD

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