Anti-Hu CD114 PE

Anti-Hu CD114 PE
Regulatory status
RUO
Antigen
CD114
Clone
LMM741
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-157-T100
In stock
220.00 USD

25 tests
1P-157-T025
Delivery 1 week
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody LMM741 recognizes an extracellular epitope of CD114 (colony stimulating factor 3 receptor), a 130 kDa transmembrane glycoprotein expressed on granulocytes and their differentiation stages, on monocytes, platelets, endothelial cells and placenta. It is absent from lymphocytes and erythrocytes.
Workshop
HLDA VI: WS Code MA98
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Negative species
Mouse
Immunogen
CHO cells transfected with human CD114
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
GCSFR, CSF3R, SCN7
Antigen description
CD114 / G-CSFR (granulocyte colony-stimulating factor receptor, also known as CSF3R) is a type I transmembrane glycoprotein which upon binding of its ligand (G-CSF, granulocyte colony-stimulating factor) homodimerizes and activates signaling transduction to mediate cell proliferation, survival, and differentiation. It is expressed by granulocytes at all stages of their differentiation, as well as by monocytes, dendritic cells, and mature platelets. Among non-hematopoietic cells, it is expressed e.g. by endothelial cells, placenta, trophoblasts, and many tumor cell lines. This antigen is a target for stem cell mobilization for blood stem cell transplantation, for enhancing recovery of myelopoiesis following chemotherapy and in the treatment of patients with severe chronic neutropenia.
Entrez Gene ID 1441
UniProt ID Q99062
1P-157 FC
Flow cytometry analysis (surface staining) of CD114 in human peripheral blood with anti-CD114 (LMM741) PE.

Product specific references:

Layton JE, Shimamoto G, Osslund T, Hammacher A, Smith DK, Treutlein HR, Boone T: Interaction of granulocyte colony-stimulating factor (G-CSF) with its receptor. Evidence that Glu19 of G-CSF interacts with Arg288 of the receptor. J Biol Chem. 1999 Jun 18;274(25):17445-51.
PubMed
Layton JE, Hall NE, Connell F, Venhorst J, Treutlein HR: Identification of ligand-binding site III on the immunoglobulin-like domain of the granulocyte colony-stimulating factor receptor. J Biol Chem. 2001 Sep 28;276(39):36779-87.
PubMed
Gibbs KD Jr, Gilbert PM, Sachs K, Zhao F, Blau HM, Weissman IL, Nolan GP, Majeti R: Single-cell phospho-specific flow cytometric analysis demonstrates biochemical and functional heterogeneity in human hematopoietic stem and progenitor compartments. Blood. 2011 Apr 21;117(16):4226-33. doi: 10.1182/blood-2010-07-298232.
PubMed
Tchou J, Zhang PJ, Bi Y, Satija C, Marjumdar R, Stephen TL, Lo A, Chen H, Mies C, June CH, Conejo-Garcia J, Puré E: Fibroblast activation protein expression by stromal cells and tumor-associated macrophages in human breast cancer. Hum Pathol. 2013 Nov;44(11):2549-57.
PubMed
Cimato TR, Palka BA, Lang JK, Young RF: LDL cholesterol modulates human CD34+ HSPCs through effects on proliferation and the IL-17 G-CSF axis. PLoS One. 2013 Aug 26;8(8):e73861.
PubMed
Layton JE, Iaria J, Smith DK, Treutlein HR: Identification of a ligand-binding site on the granulocyte colony-stimulating factor receptor by molecular modeling and mutagenesis. J Biol Chem. 1997 Nov 21;272(47):29735-41.
PubMed
Variant
100 tests
1P-157-T100
In stock
220.00 USD

25 tests
1P-157-T025
Delivery 1 week
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD

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