Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD114 APC

Anti-Hu CD114 APC

Regulatory status

RUO

Antigen

CD114

Clone

LMM741

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-157-T100

In stock

242.00 USD

Variant

100 tests

1A-157-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody LMM741 recognizes an extracellular epitope of CD114 (colony stimulating factor 3 receptor), a 130 kDa transmembrane glycoprotein expressed on granulocytes and their differentiation stages, on monocytes, platelets, endothelial cells and placenta. It is absent from lymphocytes and erythrocytes.

Workshop

HLDA VI: WS Code MA98

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Negative species

Mouse

Immunogen

CHO cells transfected with human CD114

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

GCSFR, CSF3R, SCN7

Antigen description

CD114 / G-CSFR (granulocyte colony-stimulating factor receptor, also known as CSF3R) is a type I transmembrane glycoprotein which upon binding of its ligand (G-CSF, granulocyte colony-stimulating factor) homodimerizes and activates signaling transduction to mediate cell proliferation, survival, and differentiation. It is expressed by granulocytes at all stages of their differentiation, as well as by monocytes, dendritic cells, and mature platelets. Among non-hematopoietic cells, it is expressed e.g. by endothelial cells, placenta, trophoblasts, and many tumor cell lines. This antigen is a target for stem cell mobilization for blood stem cell transplantation, for enhancing recovery of myelopoiesis following chemotherapy and in the treatment of patients with severe chronic neutropenia.

Entrez Gene ID 1441

UniProt ID Q99062

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD114 (LMM741) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD114 (LMM741) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Layton JE, Shimamoto G, Osslund T, Hammacher A, Smith DK, Treutlein HR, Boone T: Interaction of granulocyte colony-stimulating factor (G-CSF) with its receptor. Evidence that Glu19 of G-CSF interacts with Arg288 of the receptor. J Biol Chem. 1999 Jun 18;274(25):17445-51.
PubMed

Layton JE, Hall NE, Connell F, Venhorst J, Treutlein HR: Identification of ligand-binding site III on the immunoglobulin-like domain of the granulocyte colony-stimulating factor receptor. J Biol Chem. 2001 Sep 28;276(39):36779-87.
PubMed

Gibbs KD Jr, Gilbert PM, Sachs K, Zhao F, Blau HM, Weissman IL, Nolan GP, Majeti R: Single-cell phospho-specific flow cytometric analysis demonstrates biochemical and functional heterogeneity in human hematopoietic stem and progenitor compartments. Blood. 2011 Apr 21;117(16):4226-33. doi: 10.1182/blood-2010-07-298232.
PubMed

Tchou J, Zhang PJ, Bi Y, Satija C, Marjumdar R, Stephen TL, Lo A, Chen H, Mies C, June CH, Conejo-Garcia J, Puré E: Fibroblast activation protein expression by stromal cells and tumor-associated macrophages in human breast cancer. Hum Pathol. 2013 Nov;44(11):2549-57.
PubMed

Cimato TR, Palka BA, Lang JK, Young RF: LDL cholesterol modulates human CD34+ HSPCs through effects on proliferation and the IL-17 G-CSF axis. PLoS One. 2013 Aug 26;8(8):e73861.
PubMed

Layton JE, Iaria J, Smith DK, Treutlein HR: Identification of a ligand-binding site on the granulocyte colony-stimulating factor receptor by molecular modeling and mutagenesis. J Biol Chem. 1997 Nov 21;272(47):29735-41.
PubMed