Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD112 Purified

Anti-Hu CD112 Purified

Regulatory status

RUO

Antigen

CD112

Clone

R2.525

Format

Purified

Reactivity

Human

Application

IP, FC (QC tested), IHC(F)

Variant

0.1 mg

11-777-C100

In stock

130.00 USD

0.025 mg

11-777-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-777-C100

In stock

130.00 USD

0.025 mg

11-777-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody R2.525 recognizes an extracellular epitope on CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors.

Workshop

HCDM listed

Application

IP, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

NIH/3T3 cells transfected with human Nectin-2

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

NECTIN2, HVEB, PRR2, PVRL2, PVRR2

Antigen description

CD112, also known as nectin-2, is a transmembrane glycoprotein involved in organization of adherens junctions. It also serves as a target molecule for entry of certain strains of herpes simplex virus (HSV) and pseudorabies virus (PRV). It is homologous to CD155, which serves as a target molecule for polio virus. CD112 seems to play a role in neural tube formation, with N-cadherin. Inside the cell, CD112 is connected with actin cytoskeleton through afadin. Variations in the CD112 gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Entrez Gene ID 5819

UniProt ID Q92692

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD112 (R2.525) purified antibody (concentration in sample 9 µg/ml) GAM APC.

Separation of human CD112 positive thrombocytes(red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD112 (R2.525) purified antibody (concentration in sample 9 µg/ml) GAM APC.

Product specific references:

Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25.
PubMed

Hou S, Ge K, Zheng X, Wei H, Sun R, Tian Z: CD226 protein is involved in immune synapse formation and triggers Natural Killer (NK) cell activation via its first extracellular domain. J Biol Chem. 2014 Mar 7;289(10):6969-77.
PubMed

Enqvist M, Nilsonne G, Hammarfjord O, Wallin RP, Björkström NK, Björnstedt M, Hjerpe A, Ljunggren HG, Dobra K, Malmberg KJ, Carlsten M: Selenite induces posttranscriptional blockade of HLA-E expression and sensitizes tumor cells to CD94/NKG2A-positive NK cells. J Immunol. 2011 Oct 1;187(7):3546-54.
PubMed