Anti-Hu CD112 PE

Anti-Hu CD112 PE
Regulatory status
RUO
Antigen
CD112
Clone
R2.525
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-777-T100
In stock
242.00 USD

25 tests
1P-777-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-777-T100
In stock
242.00 USD

25 tests
1P-777-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody R2.525 recognizes an extracellular epitope on CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors.
Workshop
HCDM listed
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NIH/3T3 cells transfected with human Nectin-2
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
NECTIN2, HVEB, PRR2, PVRL2, PVRR2
Antigen description
CD112, also known as nectin-2, is a transmembrane glycoprotein involved in organization of adherens junctions. It also serves as a target molecule for entry of certain strains of herpes simplex virus (HSV) and pseudorabies virus (PRV). It is homologous to CD155, which serves as a target molecule for polio virus. CD112 seems to play a role in neural tube formation, with N-cadherin. Inside the cell, CD112 is connected with actin cytoskeleton through afadin. Variations in the CD112 gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Entrez Gene ID 5819
UniProt ID Q92692
1P-777_FC_Histogram
Separation of CACO-2 cells stained using anti-human CD112 (R2.525) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from CACO-2 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as CD112 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of CACO-2 cell suspension.

Product specific references:

Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25.
PubMed
Hou S, Ge K, Zheng X, Wei H, Sun R, Tian Z: CD226 protein is involved in immune synapse formation and triggers Natural Killer (NK) cell activation via its first extracellular domain. J Biol Chem. 2014 Mar 7;289(10):6969-77.
PubMed
Enqvist M, Nilsonne G, Hammarfjord O, Wallin RP, Björkström NK, Björnstedt M, Hjerpe A, Ljunggren HG, Dobra K, Malmberg KJ, Carlsten M: Selenite induces posttranscriptional blockade of HLA-E expression and sensitizes tumor cells to CD94/NKG2A-positive NK cells. J Immunol. 2011 Oct 1;187(7):3546-54.
PubMed
Variant
100 tests
1P-777-T100
In stock
242.00 USD

25 tests
1P-777-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-777-T100
In stock
242.00 USD

25 tests
1P-777-T025
Delivery 1 week
121.00 USD

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