Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD111 Purified

Anti-Hu CD111 Purified

Regulatory status

RUO

Antigen

CD111

Clone

R1.302

Format

Purified

Reactivity

Human

Application

IP, IHC, WB, FC (QC tested), ICC

Variant

0.1 mg

11-780-C100

In stock

143.00 USD

0.025 mg

11-780-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-780-C100

In stock

143.00 USD

0.025 mg

11-780-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody R1.302 recognizes an extracellular epitope on CD111 (also known as Nectin 1), a 75 kDa type I transmembrane glycoprotein broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells.

Workshop

HCDM listed

Application

IP, IHC, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

NIH/3T3 cells transfected with human CD111

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

NECTIN1, ED4, HIgR, HV1S, HVEC, OFC7, PRR1, PVRR, CD111, PVRL1, PVRR1, SK-12, CLPED1

Antigen description

CD111, also known as nectin-1, is a calcium-independent cell-cell adhesion transmembrane glycoprotein involved in organization of adherens junctions and tight junctions in epithelial and endothelial cells. It also serves as a target molecule for entry of herpes simplex virus (HSV-1, HSV-2) and pseudorabies virus (PRV) into epithelial and neuronal cells. CD111 is connected with actin cytoskeleton through afadin. Mutations in the gene for CD111 cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.

Entrez Gene ID 5818

UniProt ID Q15223

Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD111 (R1.302) purified, GAM-APC.

General references:

Cocchi F, Lopez M, Dubreuil P, Campadelli Fiume G, Menotti L: Chimeric nectin1-poliovirus receptor molecules identify a nectin1 region functional in herpes simplex virus entry. J Virol. 2001 Sep;75(17):7987-94.
PubMed

Product specific references:

Cocchi F, Lopez M, Menotti L, Aoubala M, Dubreuil P, Campadelli-Fiume G: The V domain of herpesvirus Ig-like receptor (HIgR) contains a major functional region in herpes simplex virus-1 entry into cells and interacts physically with the viral glycoprotein D.
PubMed

Yoon M, Spear PG: Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 2002 Jul;76(14):7203-8.
PubMed

Wang PY, Swain HM, Kunkler AL, Chen CY, Hutzen BJ, Arnold MA, Streby KA, Collins MH, Dipasquale B, Stanek JR, Conner J, van Kuppevelt TH, Glorioso JC, Grandi P, Cripe TP: Neuroblastomas vary widely in their sensitivities to herpes simplex virotherapy unrelated to virus receptors and susceptibility. Gene Ther. 2016 Feb;23(2):135-43.
PubMed

Reymond N, Garrido-Urbani S, Borg JP, Dubreuil P, Lopez M: PICK-1: a scaffold protein that interacts with Nectins and JAMs at cell junctions. FEBS Lett. 2005 Apr 11;579(10):2243-9.
PubMed