Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD109 Purified

Anti-Hu CD109 Purified

Regulatory status

RUO

Antigen

CD109

Clone

W7C5

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-131-C100

In stock

187.00 USD

0.025 mg

11-131-C025

Delivery 1 week

93.50 USD

Variant

0.1 mg

11-131-C100

In stock

187.00 USD

0.025 mg

11-131-C025

Delivery 1 week

93.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody W7C5 recognizes CD109, an approximately 165 kDa GPI-anchored extracellular protein expressed mainly on various hematopoietic cells, activated T lymphoblasts, activated platelets, and endothelial cells.

Workshop

HLDA VIII

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

WERI-RB-1 retinoblastoma cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CPAMD7, p180, r150, FLJ38569, GPA

Antigen description

CD109, also known as the Gov platelet alloantigen, is a GPI-anchored glycoprotein which localizes to the surface of platelets, activated T-cells, and endothelial cells, as well as of various hematopoietic cells and T cell lines. The protein binds to and negatively regulates signaling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. The Gov antigen system is involved in platelet transfusion reaction, posttransfusion purpura and in neonatal alloimmune thrombocytopenia.

Entrez Gene ID 135228

UniProt ID Q6YHK3

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD109 (W7C5) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Separation of human monocytes (red-filled) from CD109 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD109 (W7C5) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Product specific references:

Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, Andriolo G, Sun B, Zheng B, Zhang L, Norotte C, Teng PN, Traas J, Schugar R, Deasy BM, Badylak S, Buhring HJ, Giacobino JP, Lazzari L, Huard J, Péault B: A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008 Sep 11;3(3):301-13. doi: 10.1016/j.stem.2008.07.003.
PubMed

Giesert C, Marxer A, Sutherland DR, Schuh AC, Kanz L, Buhring HJ: Antibody W7C5 defines a CD109 epitope expressed on CD34+ and CD34- hematopoietic and mesenchymal stem cell subsets. Ann N Y Acad Sci. 2003 May;996:227-30.
PubMed