Anti-Hu CD109 PE

Anti-Hu CD109 PE
Regulatory status
RUO
Antigen
CD109
Clone
W7C5
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-131-T100
In stock
242.00 USD

25 tests
1P-131-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-131-T100
In stock
242.00 USD

25 tests
1P-131-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody W7C5 recognizes CD109, an approximately 165 kDa GPI-anchored extracellular protein expressed mainly on various hematopoietic cells, activated T lymphoblasts, activated platelets, and endothelial cells.
Workshop
HLDA VIII
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
WERI-RB-1 retinoblastoma cell line
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CPAMD7, p180, r150, FLJ38569, GPA
Antigen description
CD109, also known as the Gov platelet alloantigen, is a GPI-anchored glycoprotein which localizes to the surface of platelets, activated T-cells, and endothelial cells, as well as of various hematopoietic cells and T cell lines. The protein binds to and negatively regulates signaling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. The Gov antigen system is involved in platelet transfusion reaction, posttransfusion purpura and in neonatal alloimmune thrombocytopenia.
Entrez Gene ID 135228
UniProt ID Q6YHK3
1P-131_FC_Profil Značení
Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD109 (W7C5) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
1P-131_FC_Histogram
Separation of human CD109 positive cells (red-filled) from CD109 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD109 (W7C5) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Product specific references:

Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, Andriolo G, Sun B, Zheng B, Zhang L, Norotte C, Teng PN, Traas J, Schugar R, Deasy BM, Badylak S, Buhring HJ, Giacobino JP, Lazzari L, Huard J, Péault B: A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008 Sep 11;3(3):301-13. doi: 10.1016/j.stem.2008.07.003.
PubMed
Giesert C, Marxer A, Sutherland DR, Schuh AC, Kanz L, Buhring HJ: Antibody W7C5 defines a CD109 epitope expressed on CD34+ and CD34- hematopoietic and mesenchymal stem cell subsets. Ann N Y Acad Sci. 2003 May;996:227-30.
PubMed
Variant
100 tests
1P-131-T100
In stock
242.00 USD

25 tests
1P-131-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-131-T100
In stock
242.00 USD

25 tests
1P-131-T025
Delivery 1 week
121.00 USD

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