Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD107b Purified Azide Free

Anti-Hu CD107b Purified Azide Free

Regulatory status

RUO

Antigen

CD107b

Clone

H4B4

Format

Purified Azide Free

Reactivity

Human

Application

WB, FC (QC tested), IHC-F, IHC-P, ICC

Variant

0.1 mg

10-672-C100

In stock

147.40 USD

0.025 mg

10-672-C025

Delivery 1 week

73.70 USD

Variant

0.1 mg

10-672-C100

In stock

147.40 USD

0.025 mg

10-672-C025

Delivery 1 week

73.70 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody H4B4 recognizes an extracellular/luminal epitope of CD107b / LAMP-2, an extensively glycosylated 100-120 kDa widely expressed lysosome-associated protein.

Workshop

HLDA V: WS Code P007

Application

WB, FC (QC tested), IHC-F, IHC-P, ICC

Application details

Flow cytometry: Intracellular and extracellular staining; recommended dilution: 1-2 μg/ml.
Immunohistochemistry: Recommended dilution: 2-8 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Human

Negative species

Mouse, Rat

Immunogen

Human PBMC

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LAMP-2, LAMPB

Antigen description

CD107b (lysosome-associated membrane protein-2, LAMP-2), together with CD107a / LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, especially CD107b is important regulator in successful phagosomal maturation. CD107b deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danons disease). Immature CD107b is an approximately 45 kDa protein, but after extensive glycosylation the mature glycoprotein has about 100-120 kDa.

Entrez Gene ID 3920

UniProt ID P13473

Flow cytometry surface staining pattern of human anti-IgE antibody stimulated peripheral whole blood stained using anti-human CD107b (H4B4) purified antibody (concentration in sample 1,67 μg/ml, GAM APC).

Flow cytometry multicolor surface staining of human anti-IgE antibody stimulated mononuclear cells stained using anti-human CD107b (H4B4) purified antibody (concentration in sample 1,67 μg/ml, GAM APC) and anti-human CD203c (NP4D6) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD107b positive CD203c positive basophil granulocytes (red-filled) from CD107b negative CD203c positive basophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human anti-IgE antibody stimulated peripheral whole blood stained using anti-human CD107b (H4B4) purified antibody (concentration in sample 1,67 μg/ml, GAM APC).

Anti-Hu CD107b Purified (clone H4B4) works in WB application under reducing and non-reducing conditions.

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of SK-MEL30, MCF-7, THP-1, and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody H4B4 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml) was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.

CD107b molecules were detected at ~95-125 kDa in all tested cell lines under both reducing and non-reducing conditions.

Product specific references:

Apte SH, Baz A, Groves P, Kelso A, Kienzle N: Interferon-gamma and interleukin-4 reciprocally regulate CD8 expression in CD8+ T cells. Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17475-80.
PubMed

Palmer DR, Fernandez S, Bisbing J, Peachman KK, Rao M, Barvir D, Gunther V, Burgess T, Kohno Y, Padmanabhan R, Sun W: Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells. J Gen Virol. 2007 Jan;88(Pt 1):148-56.
PubMed

Denzer K, van Eijk M, Kleijmeer MJ, Jakobson E, de Groot C, Geuze HJ: Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface. J Immunol. 2000 Aug 1;165(3):1259-65.
PubMed

Thedrez A, Harly C, Morice A, Salot S, Bonneville M, Scotet E: IL-21-mediated potentiation of antitumor cytolytic and proinflammatory responses of human V gamma 9V delta 2 T cells for adoptive immunotherapy. J Immunol. 2009 Mar 15;182(6):3423-31.
PubMed

Kannanganat S, Ibegbu C, Chennareddi L, Robinson HL, Amara RR: Multiple-cytokine-producing antiviral CD4 T cells are functionally superior to single-cytokine-producing cells. J Virol. 2007 Aug;81(16):8468-76.
PubMed

Guia S, Cognet C, de Beaucoudrey L, Tessmer MS, Jouanguy E, Berger C, Filipe-Santos O, Feinberg J, Camcioglu Y, Levy J, Al Jumaah S, Al-Hajjar S, Stephan JL, Fieschi C, Abel L, Brossay L, Casanova JL, Vivier E: A role for interleukin-12/23 in the maturation of human natural killer and CD56+ T cells in vivo. Blood. 2008 May 15;111(10):5008-16.
PubMed

Meade JL, Wilson EB, Holmes TD, de Wynter EA, Brett P, Straszynski L, Ballard PA, Trapani JA, McDermott MF, Cook GP: Proteolytic activation of the cytotoxic phenotype during human NK cell development. J Immunol. 2009 Jul 15;183(2):803-13.
PubMed

Borgne-Sanchez A, Dupont S, Langonné A, Baux L, Lecoeur H, Chauvier D, Lassalle M, Déas O, Brière JJ, Brabant M, Roux P, Péchoux C, Briand JP, Hoebeke J, Deniaud A, Brenner C, Rustin P, Edelman L, Rebouillat D, Jacotot E: Targeted Vpr-derived peptides reach mitochondria to induce apoptosis of alphaVbeta3-expressing endothelial cells. Cell Death Differ. 2007 Mar;14(3):422-35.
PubMed