Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD107a Purified Azide Free

Anti-Hu CD107a Purified Azide Free

Regulatory status

RUO

Antigen

CD107a

Clone

H4A3

Format

Purified Azide Free

Reactivity

Human, Non-human primates, Mouse

Application

WB, FC (QC tested), IHC-F, IHC-P, ICC

Variant

0.1 mg

10-671-C100

In stock

143.00 USD

0.025 mg

10-671-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

10-671-C100

In stock

143.00 USD

0.025 mg

10-671-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody H4A3 recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.

Workshop

HLDA V

Application

WB, FC (QC tested), IHC-F, IHC-P, ICC

Application details

Western blotting: Recommended dilution: 1 μg/ml.
Immunohistochemistry: Recommended dilution: 2-8 μg/ml.
Flow cytometry: Intracellular and extracellular staining; recommended dilution: 1-12 µg/ml.

Reactivity

Human, Non-human primates, Mouse

Immunogen

Human PBMC

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LAMP-1, LAMPA

Antigen description

CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.

Entrez Gene ID 3916

UniProt ID P11279

Immunohistochemistry staining of CD107a (red) in tissue sections of murine brain expressing GFP in some of its neurons (green). Mouse monoclonal antibody H4A3 (anti-CD107a) was detected with goat anti-mouse IgG1 conjugated with Alexa Fluor 555.

Anti-Hu CD107a Purified (clone H4A3) works in WB application under reducing and non-reducing conditions.

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of A431, THP-1, U87MG, and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody H4A3 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.

CD107a molecules were detected at 120 kDa in all tested cell lines under both reducing and non-reducing conditions.

Flow cytometry analysis (intracellular staining) of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.

General references:

Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8.
PubMed

Barrachina M, Maes T, Buesa C, Ferrer I: Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 2006 Oct;32(5):505-16.
PubMed

Sarafian VS, Marinova TT: Lysosomal membrane-associated glycoproteins are differentially expressed in acute and chronic human thymic involution. Acta Biol Hung. 2006 Sep;57(3):315-22.
PubMed

Eskelinen EL: Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Mol Aspects Med. 2006 Oct-Dec;27(5-6):495-502.
PubMed

Hunyh KK, Eskelinen EL, Scott CC, Malevanets A, Saftig P, Grinstein S: LAMP proteins are required for fusion of lysosomes with phagosomes. EMBO J. 2007 Jan 24;26(2):313-24.
PubMed

Product specific references:

Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95.
PubMed

Mao H, Tu W, Liu Y, Qin G, Zheng J, Chan PL, Lam KT, Peiris JS, Lau YL: Inhibition of human natural killer cell activity by influenza virions and hemagglutinin. J Virol. 2010 May;84(9):4148-57.
PubMed

Yu CI, Gallegos M, Marches F, Zurawski G, Ramilo O, García-Sastre A, Banchereau J, Palucka AK: Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines. Blood. 2008 Nov 1;112(9):3671-8.
PubMed

Tomescu C, Chehimi J, Maino VC, Montaner LJ: NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells. J Immunol. 2007 Aug 15;179(4):2097-104.
PubMed

Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25.
PubMed

Marcenaro S, Gallo F, Martini S, Santoro A, Griffiths GM, Aricó M, Moretta L, Pende D: Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease. Blood. 2006 Oct 1;108(7):2316-23.
PubMed

Deetz CO, Hebbeler AM, Propp NA, Cairo C, Tikhonov I, Pauza CD: Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys. Infect Immun. 2006 Aug;74(8):4505-11.
PubMed

Furuta K, Ikeda M, Nakayama Y, Nakamura K, Tanaka M, Hamasaki N, Himeno M, Hamilton SR, August JT: Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases. Am J Pathol. 2001 Aug;159(2):449-55.
PubMed

Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, August JT: Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 1989 Jan;268(1):360-78.
PubMed