Anti-Hu CD107a PE

Anti-Hu CD107a PE
Regulatory status
RUO
Antigen
CD107a
Clone
H4A3
Format
PE
Reactivity
Human, Non-human primates, Mouse
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-671-T100
In stock
242.00 USD

25 tests
1P-671-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-671-T100
In stock
242.00 USD

25 tests
1P-671-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody H4A3 recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
Workshop
HLDA V
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular and extracellular staining.
Reactivity
Human, Non-human primates, Mouse
Immunogen
Human PBMC
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LAMP-1, LAMPA
Antigen description
CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Entrez Gene ID 3916
UniProt ID P11279
1P-671_FC_Profil Značení
Flow cytometry surface staining pattern of anti-IgE stimulated human peripheral whole blood stained using anti-human CD107a (H4A3) PE antibody (concentration in sample 3 μg/ml).
1P-671_FC_CDMaps-histogram-adapt
Expression profiling on peripheral blood subsets using Anti-human CD107a PE antibody (clone H4A3). Adaptive panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human CD107a PE antibody (clone H4A3, 8 µg/ml in stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Adaptive) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.
1P-671_FC_CDMaps-histogram-adaptive-intracel
Expression profiling on peripheral blood subsets using Anti-human CD107a PE antibody (clone H4A3). Intracellular staining, adaptive panel

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm buffer set (#ED7397).
HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for leukocyte isolation.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of optimized backbone antibody panel (HLDA Adaptive) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min.
Next, EXCELLYSE XPerm buffer set (#ED7397) was used for leukocytes permeabilization according to the instruction procedure.
Mouse monoclonal anti-human CD107a PE antibody (clone H4A3) was used in concentration 8 µg/ml in stained blood sample (2 x 106 cells).
1P-671_FC_CDMaps-histogram-innate
Expression profiling on peripheral blood subsets using Anti-human CD107a PE antibody (clone H4A3). Innate panel

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human CD107a PE antibody (clone H4A3, 8 µg/ml in stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Innate) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.
1P-671_FC_CDMaps-histogram-innate-intra
Expression profiling on peripheral blood subsets using Anti-human CD107a PE antibody (clone H4A3). Intracellular staining, innate panel

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm buffer set (#ED7397).
HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for leukocyte isolation.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of optimized backbone antibody panel (HLDA Innate) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min.
Next, EXCELLYSE XPerm buffer set (#ED7397) was used for leukocytes permeabilization according to the instruction procedure.
Mouse monoclonal anti-human CD107a PE antibody (clone H4A3) was used in concentration 8 µg/ml in stained blood sample (2 x 106 cells).
1P-671_FC_CDMaps-profil značení
Anti-human CD107a PE antibody (clone H4A3) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Mouse monoclonal anti-human CD107a PE antibody (clone H4A3) was used in concentration 8 µg/ml in stained blood sample (2 x 106 cells).
1P-671_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of anti-IgE stimulated human peripheral blood mononuclear cells stained using anti-human CD203c (NP4D6) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD107a (H4A3) PE antibody (concentration in sample 3 μg/ml).
1P-671_FC_Histogram
Separation of human CD107a positive CD203c positive basophils (red-filled) from neutrophils granulocytes (black-dashed) in flow cytometry analysis (surface staining) of anti-IgE stimulated human peripheral whole blood stained using anti-human CD107a (H4A3) PE antibody (concentration in sample 3 μg/ml).

General references:

Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8.
PubMed
Barrachina M, Maes T, Buesa C, Ferrer I: Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 2006 Oct;32(5):505-16.
PubMed
Sarafian VS, Marinova TT: Lysosomal membrane-associated glycoproteins are differentially expressed in acute and chronic human thymic involution. Acta Biol Hung. 2006 Sep;57(3):315-22.
PubMed
Eskelinen EL: Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Mol Aspects Med. 2006 Oct-Dec;27(5-6):495-502.
PubMed
Hunyh KK, Eskelinen EL, Scott CC, Malevanets A, Saftig P, Grinstein S: LAMP proteins are required for fusion of lysosomes with phagosomes. EMBO J. 2007 Jan 24;26(2):313-24.
PubMed

Product specific references:

Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95.
PubMed
Mao H, Tu W, Liu Y, Qin G, Zheng J, Chan PL, Lam KT, Peiris JS, Lau YL: Inhibition of human natural killer cell activity by influenza virions and hemagglutinin. J Virol. 2010 May;84(9):4148-57.
PubMed
Yu CI, Gallegos M, Marches F, Zurawski G, Ramilo O, García-Sastre A, Banchereau J, Palucka AK: Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines. Blood. 2008 Nov 1;112(9):3671-8.
PubMed
Tomescu C, Chehimi J, Maino VC, Montaner LJ: NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells. J Immunol. 2007 Aug 15;179(4):2097-104.
PubMed
Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25.
PubMed
Marcenaro S, Gallo F, Martini S, Santoro A, Griffiths GM, Aricó M, Moretta L, Pende D: Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease. Blood. 2006 Oct 1;108(7):2316-23.
PubMed
Deetz CO, Hebbeler AM, Propp NA, Cairo C, Tikhonov I, Pauza CD: Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys. Infect Immun. 2006 Aug;74(8):4505-11.
PubMed
Furuta K, Ikeda M, Nakayama Y, Nakamura K, Tanaka M, Hamasaki N, Himeno M, Hamilton SR, August JT: Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases. Am J Pathol. 2001 Aug;159(2):449-55.
PubMed
Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, August JT: Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 1989 Jan;268(1):360-78.
PubMed
Variant
100 tests
1P-671-T100
In stock
242.00 USD

25 tests
1P-671-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-671-T100
In stock
242.00 USD

25 tests
1P-671-T025
Delivery 1 week
121.00 USD

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